BRENDA - Enzyme Database
show all sequences of 1.13.11.66

Cloning and characterization of a gene cluster involved in the catabolism of p-nitrophenol from Pseudomonas putida DLL-E4

Shen, W.; Liu, W.; Zhang, J.; Tao, J.; Deng, H.; Cao, H.; Cui, Z.; Biores. Technol. 101, 7516-7522 (2010)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
additional information
expression of the hydroquinone dioxygenase PnpC1C2 multi-component protein complex in Escherichia coli results in conversion of hydroquinone to 4-hydroxymuconic semialdehyde
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
x * 19000, subunit PnpC1
Pseudomonas putida
38000
-
x * 38000, subunit PnpC2
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas putida
C6FI40
subunit C1, part of the PnpC1C2 multi-component protein complex
-
Pseudomonas putida
C6FI41
subunit C2, part of the PnpC1C2 multi-component protein complex
-
Pseudomonas putida DLL-E4
C6FI40
subunit C1, part of the PnpC1C2 multi-component protein complex
-
Pseudomonas putida DLL-E4
C6FI41
subunit C2, part of the PnpC1C2 multi-component protein complex
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
hydroquinone + O2
-
719104
Pseudomonas putida
gamma-hydroxymuconic acid semialdehyde
-
-
-
?
hydroquinone + O2
-
719104
Pseudomonas putida DLL-E4
gamma-hydroxymuconic acid semialdehyde
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 19000, subunit PnpC1
Pseudomonas putida
?
x * 38000, subunit PnpC2
Pseudomonas putida
Synonyms
Synonyms
Commentary
Organism
PnpC1
-
Pseudomonas putida
PnpC2
-
Pseudomonas putida
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
expression of the hydroquinone dioxygenase PnpC1C2 multi-component protein complex in Escherichia coli results in conversion of hydroquinone to 4-hydroxymuconic semialdehyde
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
x * 19000, subunit PnpC1
Pseudomonas putida
38000
-
x * 38000, subunit PnpC2
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
hydroquinone + O2
-
719104
Pseudomonas putida
gamma-hydroxymuconic acid semialdehyde
-
-
-
?
hydroquinone + O2
-
719104
Pseudomonas putida DLL-E4
gamma-hydroxymuconic acid semialdehyde
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 19000, subunit PnpC1
Pseudomonas putida
?
x * 38000, subunit PnpC2
Pseudomonas putida
Other publictions for EC 1.13.11.66
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744430
Ferraroni
The crystal structures of nat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
Biochim. Biophys. Acta
1865
520-530
2017
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-
-
1
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-
2
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1
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2
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8
-
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1
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6
2
2
1
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1
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6
2
1
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1
-
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1
1
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-
745331
Liu
Crystal structure of PnpCD, a ...
Pseudomonas sp.
J. Biol. Chem.
290
24547-24560
2015
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-
1
1
9
-
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3
-
2
-
1
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1
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2
2
2
1
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1
9
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3
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2
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1
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2
2
1
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1
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3
3
-
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745802
Mancini
A copper-induced quinone degr ...
Lactococcus lactis, Lactococcus lactis IL1403
Mol. Microbiol.
95
645-659
2015
-
-
1
-
-
1
1
1
-
2
-
6
-
13
-
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1
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11
1
3
1
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1
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1
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1
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1
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1
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2
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6
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1
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-
11
1
1
-
-
-
1
-
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-
1
1
1
1
-
-
719179
Zhang
Identification of the para-nit ...
Pseudomonas sp.
BMC Microbiol.
12
27
2012
-
-
1
-
-
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-
-
-
-
1
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8
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1
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2
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2
1
1
1
-
1
1
2
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1
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1
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1
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2
-
1
1
1
-
1
1
2
-
-
-
-
-
-
-
718555
Kolvenbach
Purification and characterizat ...
Sphingomonas sp., Sphingomonas sp. TTNP3
AMB Express
1
08
2011
-
-
-
-
-
-
9
2
-
1
6
-
-
9
-
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2
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-
-
2
-
32
2
-
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-
2
-
2
-
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18
-
2
-
1
6
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2
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2
-
32
2
-
-
-
2
-
2
-
-
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-
-
-
2
2
718916
Machonkin
Substrate specificity of Sphin ...
Sphingobium chlorophenolicum
Biochemistry
50
8899-8913
2011
-
-
-
-
-
-
3
12
-
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-
1
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8
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9
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3
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12
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-
8
-
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-
9
-
-
-
-
-
-
-
-
9
9
719104
Shen
Cloning and characterization o ...
Pseudomonas putida, Pseudomonas putida DLL-E4
Biores. Technol.
101
7516-7522
2010
-
-
-
-
2
-
-
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2
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7
-
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4
2
2
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2
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2
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4
2
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-
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-
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-
719327
Yin
Characterization of MnpC, a hy ...
Cupriavidus necator, Cupriavidus necator JMP 134-1
Curr. Microbiol.
61
471-476
2010
-
-
1
-
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1
-
2
1
-
-
28
-
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1
3
4
1
1
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1
1
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1
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1
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2
1
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1
3
4
1
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1
1
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720066
Machonkin
Determination of the active si ...
Sphingobium chlorophenolicum
J. Biol. Inorg. Chem.
15
291-301
2010
-
-
1
1
5
-
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-
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1
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1
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1
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1
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1
5
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1
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719714
Moonen
Hydroquinone dioxygenase from ...
Pseudomonas fluorescens, Pseudomonas fluorescens ACB
J. Bacteriol.
190
5199-5209
2008
1
-
-
-
-
-
12
1
-
1
3
-
-
13
-
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1
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1
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14
1
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1
1
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1
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1
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12
1
1
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1
3
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1
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1
-
14
1
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1
1
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-
718727
Xun
Characterization of 2,6-dichlo ...
Sphingobium chlorophenolicum
Biochem. Biophys. Res. Commun.
266
322-325
1999
-
-
1
-
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-
1
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1
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1
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1
1
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1
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1
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2
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1
1
1
-
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-
718831
Xu
Evidence that pcpA encodes 2,6 ...
Sphingobium chlorophenolicum
Biochemistry
38
7659-7669
1999
-
-
-
-
-
-
-
-
-
2
1
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1
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1
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1
1
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1
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1
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1
1
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-
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-
719693
Miyauchi
Cloning and sequencing of a no ...
Sphingomonas paucimobilis
J. Bacteriol.
181
6712-6719
1999
-
-
1
-
3
-
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1
1
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1
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2
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3
1
1
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1
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3
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1
1
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1
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