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Literature summary for 1.13.11.65 extracted from

  • Yao, X.; Lv, Y.; Yu, H.; Cao, H.; Wang, L.; Wen, B.; Gu, T.; Wang, F.; Sun, L.; Xin, F.
    Site-directed mutagenesis of coenzyme-independent carotenoid oxygenase CSO2 to enhance the enzymatic synthesis of vanillin (2020), Appl. Microbiol. Biotechnol., 104, 3897-3907 .
    View publication on PubMed
Search for more literature for 1.13.11.65

Activating Compound

Activating Compound Comment Organism Structure
glycerol 10% (v/v) glycerol slightly (1.2fold) stimulates vanillin production Caulobacter segnis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli T7 Express cells Caulobacter segnis

Protein Variants

Protein Variants Comment Organism
A49P the catalytic efficiency of the mutant is 1.6fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type. Under temperatures lower than 30°C, mutant A49P retains almost all the activities within the shorter incubation time (less than 5 h). The mutant also shows better performance even at 35°C Caulobacter segnis
I234A the mutant shows strongly decreased catalytic efficiency compared to the wild type enzyme Caulobacter segnis
Q390A the catalytic efficiency of the mutant is 1.64fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type Caulobacter segnis

Inhibitors

Inhibitors Comment Organism Structure
Triton X-100 0.1% (v/v) Triton X-100 significantly inhibits vanillin production (to less than 40%) Caulobacter segnis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.6 mM used in assay conditions Caulobacter segnis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-vinylguaiacol + O2 Caulobacter segnis
-
 vanillin
-
 ?
4-vinylguaiacol + O2 Caulobacter segnis ATCC 21756
-
 vanillin
-
 ?

Organism

Organism UniProt Comment Textmining
Caulobacter segnis D5VGA0
-
-
Caulobacter segnis ATCC 21756 D5VGA0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Caulobacter segnis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-vinylguaiacol + O2
-
 Caulobacter segnis vanillin
-
 ?
4-vinylguaiacol + O2
-
 Caulobacter segnis ATCC 21756 vanillin
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Caulobacter segnis

Synonyms

Synonyms Comment Organism
carotenoid cleavage oxygenase
-
 Caulobacter segnis
CSO2
-
 Caulobacter segnis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10 15
-
 Caulobacter segnis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 30 more than 80% activity between 5 and 30°C. Less than 10% of the maximum activity is retained when the temperature is higher than 30°C Caulobacter segnis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15 35 the enzyme retains about 70-90% activity after approximately 3 h at 15-23°C. At 30°C, the enzyme maintains about 50% activity after 3 h incubation. The enzyme is inactivated after about 3 h at 35°C Caulobacter segnis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Caulobacter segnis

pH Range

pH Minimum pH Maximum Comment Organism
6 10 more than 80% activity between pH 6.0 and 10.0. Less than 10% of the maximum activity is retained when the pH is lower than 6.0 Caulobacter segnis