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Literature summary for 1.13.11.65 extracted from

  • Oberhauser, V.; Voolstra, O.; Bangert, A.; von Lintig, J.; Vogt, K.
    NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide (2008), Proc. Natl. Acad. Sci. USA, 105, 19000-19005.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Drosophila melanogaster
expression in Escherichia coli Galleria mellonella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0104
-
beta,beta-carotene pH 6.8, 20°C Galleria mellonella
0.0221
-
alpha-carotene pH 6.8, 20°C Galleria mellonella

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9VFS2
-
-
Galleria mellonella A8Y9I2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-carotene + O2
-
Galleria mellonella ?
-
?
beta,beta-carotene + O2
-
Drosophila melanogaster all-trans-retinal + 11-cis-retinal
-
?
beta,beta-carotene + O2
-
Galleria mellonella all-trans-retinal + 11-cis-retinal enzyme catalyzes the production of 11-cis-retinal and all-trans-retinal, i.e. cleavage at the 15,15' double bond. The 11-cis-retinal product maybe thermally converted either into the all-trans or the 13-cis stereoisomer in the aqueous test solution ?
cryptoxanthin + O2
-
Galleria mellonella 11-cis-3-hydroxyretinal + 11-cis-retinal 11-cis-3-hydroxyretinal and 11-cis-retinal exist in a molar ratio of 8:1, indicating that the enzyme preferentially isomerizes the half site of cryptoxanthin with the hydroxylated beta-ionone ring ?
lutein + O2
-
Galleria mellonella ?
-
?
additional information enzyme combines isomerase and isomerooxygenase activity in a single polypeptide Drosophila melanogaster ?
-
?
additional information enzyme combines isomerase and isomerooxygenase activity in a single polypeptide Galleria mellonella ?
-
?
zeaxanthin + O2
-
Galleria mellonella (3R)-all-trans-3-hydroxyretinal + (3R)-11-cis-3-hydroxyretinal
-
?

Synonyms

Synonyms Comment Organism
NinaB
-
Drosophila melanogaster
NinaB
-
Galleria mellonella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0082
-
alpha-carotene pH 6.8, 20°C Galleria mellonella
0.0087
-
beta,beta-carotene pH 6.8, 20°C Galleria mellonella