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Literature summary for 1.13.11.63 extracted from
- In vitro characterization of a recombinant Blh protein from an uncultured marine bacterium as a beta-carotene 15,15-dioxygenase (2009), J. Biol. Chem., 284, 15781-15793.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | uncultured marine bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Phenanthroline | 2% residual activity | uncultured marine bacterium |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.037 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.147 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.169 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.382 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | addition of 10 microM Fe2+ after the removal of all metal ions induces a 54% recovery of the active form of the enzyme. Optimal concentration is 10 microM, regardless of phenanthroline treatment | uncultured marine bacterium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | uncultured marine bacterium |
| 64000 | - |
gel fitlration | uncultured marine bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured marine bacterium | Q4PNI0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant protein | uncultured marine bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-carotene + O2 | - |
uncultured marine bacterium | ? | - |
? | |
| beta-apo-4'-carotenal + O2 | - |
uncultured marine bacterium | ? | - |
? | |
| beta-carotene + O2 | - |
uncultured marine bacterium | 2 all-trans-retinal | the oxygen atom of retinal originates from molecular oxygen | ? | |
| beta-cryptoxanthin + O2 | - |
uncultured marine bacterium | retinal + (3R)-3-hydroxyretinal | - |
? | |
| gamma-carotene + O2 | - |
uncultured marine bacterium | ? | - |
? | |
| additional information | no substrate: beta-apo-8'-carotenal, beta-apo-12'-carotenal, lutein, zeaxanthin, or lycopene, suggesting that the presence of one unsubstituted-ionone ring in a substrate with a molecular weight greater than C35 seems to be essential for enzyme activity | uncultured marine bacterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32000, SDS-PAGE | uncultured marine bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
uncultured marine bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
15% of maximum activity | uncultured marine bacterium |
| 50 | - |
66% of maximum activity | uncultured marine bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
half-life 17.6 min | uncultured marine bacterium |
| 40 | - |
half-life 15 min | uncultured marine bacterium |
| 45 | - |
half-life 12.5 min | uncultured marine bacterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.001 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.01 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.013 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.037 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
uncultured marine bacterium |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
gamma-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.06 | - |
alpha-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.07 | - |
beta-apo-4'-carotenal | pH 8.0, 40°C | uncultured marine bacterium | |
| 0.47 | - |
beta-cryptoxanthin | pH 8.0, 40°C | uncultured marine bacterium | |
| 1.62 | - |
beta-carotene | pH 8.0, 40°C | uncultured marine bacterium | |
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