Reaction mechanism of 5,8-linoleate diol synthase, 10R-dioxygenase, and 8,11-hydroperoxide isomerase of Aspergillus clavatus

Jernerén, F.; Garscha, U.; Hoffmann, I.; Hamberg, M.; Oliw, E.H.; Biochim. Biophys. Acta 1801, 503-507 (2010)

Data extracted from this reference:

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

linoleate + O2

Aspergillus clavatus

the enzyme is involved in biosynthesis of oxylipins

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

linoleate + O2

the enzyme is involved in biosynthesis of oxylipins

714317

Aspergillus clavatus

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

-

?

linoleate + O2

abstraction of the pro-S hydrogen at C-8 and antarafacial dioxygenation at C-10 with double bond migration

714317

Aspergillus clavatus

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

-

?

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

linoleate + O2

Aspergillus clavatus

the enzyme is involved in biosynthesis of oxylipins

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

linoleate + O2

the enzyme is involved in biosynthesis of oxylipins

714317

Aspergillus clavatus

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

-

?

linoleate + O2

abstraction of the pro-S hydrogen at C-8 and antarafacial dioxygenation at C-10 with double bond migration

714317

Aspergillus clavatus

(8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

744123

Han

Production of 10R-hydroxy uns ...

Aspergillus nidulans, Aspergillus nidulans ATCC 10074

Appl. Microbiol. Biotechnol.

100

7933-7944

2016

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1

1

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1

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1

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4

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13

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3

1

1

1

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1

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1

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13

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1

1

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1

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1

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714317

Jernerén

Reaction mechanism of 5,8-lino ...

Aspergillus clavatus

Biochim. Biophys. Acta

1801

503-507

2010

-

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7

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704475

Garscha

Leucine/valine residues direct ...

Aspergillus fumigatus

J. Biol. Chem.

284

13755-13765

2009

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