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Literature summary for 1.13.11.61 extracted from
- Selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by a lipoxygenase from Nostoc sp. SAG 25.82 (2015), PLoS ONE, 10, e0137785 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetone | activates best at 5% v/v | Nostoc sp. |  |
| additional information | no effect by Brij58 | Nostoc sp. | |
| Tween 20 | - |
Nostoc sp. | |
| Tween 40 | - |
Nostoc sp. | |
| Tween 80 | activates best at 0.2% w/v | Nostoc sp. | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene 9R-lox, recombinant expression of His-tagged enzyme in Escherichia coli | Nostoc sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by Brij58; no effects by Brij58 | Nostoc sp. | |
| SDS | - |
Nostoc sp. | |
| SPAN 20 | slight inhibition | Nostoc sp. | |
| Span 40 | slight inhibition | Nostoc sp. | |
| Span 60 | slight inhibition | Nostoc sp. | |
| SPAN 80 | slight inhibition | Nostoc sp. | |
| Triton X-100 | - |
Nostoc sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Nostoc sp. | |
| 0.054 | - |
alpha-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 0.054 | - |
gamma-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 0.404 | - |
linoleic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 156000 | - |
recombinant His-tagged enzyme, gel filtration | Nostoc sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenic acid + O2 | Nostoc sp. | - |
(9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid | - |
? | |
| alpha-linolenic acid + O2 | Nostoc sp. SAG 25.82 | - |
(9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid | - |
? | |
| gamma-linolenic acid + O2 | Nostoc sp. | - |
(6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid | - |
? | |
| gamma-linolenic acid + O2 | Nostoc sp. SAG 25.82 | - |
(6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid | - |
? | |
| linoleic acid + O2 | Nostoc sp. | - |
(9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid | - |
? | |
| linoleic acid + O2 | Nostoc sp. SAG 25.82 | - |
(9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nostoc sp. | - |
- |
- |
| Nostoc sp. SAG 25.82 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Nostoc sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
recombinant enzyme, pH 8.5, 15°C, substrate gamma-linolenic acid | Nostoc sp. |
| 6.3 | - |
recombinant enzyme, pH 8.5, 15°C, substrate alpha-linolenic acid | Nostoc sp. |
| 18.4 | - |
recombinant enzyme, pH 8.5, 15°C, substrate linolenic acid | Nostoc sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-linolenic acid + O2 | - |
Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid | - |
? | |
| alpha-linolenic acid + O2 | - |
Nostoc sp. SAG 25.82 | (9R,10E,12Z,15Z)-9-hydroxy-10,12,15-octadecatrienoic acid | - |
? | |
| alpha-linolenic acid + O2 | optimal reaction conditions are pH 8.5, 15°C, 5% v/v acetone, 0.2% w/v Tween 80, 40 g/l alpha-linolenic acid, and 1 g/l enzyme, assay method optimization | Nostoc sp. | (9R,10E,12Z,15Z)-9-hydroxy-octadeca-10,12,15-trienoic acid | - |
? | |
| alpha-linolenic acid + O2 | optimal reaction conditions are pH 8.5, 15°C, 5% v/v acetone, 0.2% w/v Tween 80, 40 g/l alpha-linolenic acid, and 1 g/l enzyme, assay method optimization | Nostoc sp. SAG 25.82 | (9R,10E,12Z,15Z)-9-hydroxy-octadeca-10,12,15-trienoic acid | - |
? | |
| gamma-linolenic acid + O2 | - |
Nostoc sp. | (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid | - |
? | |
| gamma-linolenic acid + O2 | - |
Nostoc sp. SAG 25.82 | (6Z,9R,10E,12Z)-9-hydroxy-octadeca-6,10,12-trienoic acid | - |
? | |
| linoleic acid + O2 | - |
Nostoc sp. | (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid | - |
? | |
| linoleic acid + O2 | - |
Nostoc sp. SAG 25.82 | (9R,10E,12Z)-9-hydroxy-octadeca-10,12-dienoic acid | - |
? | |
| additional information | selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by the enzyme from Nostoc sp. strain SAG 25.82, heterologously expressed in Escherichia coli. The specific activity of the enzyme followed the descending order linoleic acid > alpha-linolenic acid > gamma-linolenic acid. Product determination by LC-MS/MS analysis | Nostoc sp. | ? | - |
? | |
| additional information | selective production of 9R-hydroxy-10E,12Z,15Z-octadecatrienoic acid from alpha-linolenic acid in Perilla seed oil hydrolyzate by the enzyme from Nostoc sp. strain SAG 25.82, heterologously expressed in Escherichia coli. The specific activity of the enzyme followed the descending order linoleic acid > alpha-linolenic acid > gamma-linolenic acid. Product determination by LC-MS/MS analysis | Nostoc sp. SAG 25.82 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 52000, recombinant His-tagged enzyme, SDS-PAGE, 3 * 52300, about, sequence calculation | Nostoc sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 9R-lipoxygenase | - |
Nostoc sp. |
| 9R-LOX | - |
Nostoc sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Nostoc sp. |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
gamma-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 0.13 | - |
alpha-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 5.12 | - |
linoleic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Nostoc sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 10.5 | 50% of maximal activity at pH 4.5-6.5, maximal activity at pH 8.5 | Nostoc sp. |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.82 | - |
gamma-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 2.4 | - |
alpha-linolenic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
| 12.67 | - |
linoleic acid | recombinant enzyme, pH 8.5, 15°C | Nostoc sp. | |
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