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Literature summary for 1.13.11.60 extracted from
- 7,8- and 5,8-linoleate diol synthases support the heterolytic scission of oxygen-oxygen bonds by different amide residues (2013), Arch. Biochem. Biophys., 539, 87-91.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Aspergillus fumigatus |
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Gaeumannomyces graminis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N887L | site-directed mutagenesis, the mutant retains (5S,8R)-5,8-dihydroxylinoleate as the main metabolite with an increased formation of 6,8- and 8,11-dihydroxylinoleate | Aspergillus fumigatus |
| N887Q | site-directed mutagenesis, the mutant retains (5S,8R)-5,8-dihydroxylinoleate as the main metabolite with an increased formation of 6,8- and 8,11-dihydroxylinoleate | Aspergillus fumigatus |
| N938D | site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase reduces the hydroperoxide isomerase activity of the enzyme | Gaeumannomyces graminis |
| N938L | site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase abolishes the hydroperoxide isomerase activity of the enzyme | Gaeumannomyces graminis |
| N938Q | site-directed mutagenesis, the mutation of the 7,8-linoleate diol synthase reduces the hydroperoxide isomerase activity of the enzyme | Gaeumannomyces graminis |
| Q890E | site-directed mutagenesis, the mutant retains (5S,8R)-5,8-dihydroxylinoleate as the main product, but shifts oxygenation from C-5 to C-7 and C-11 and to formation of epoxyalcohols by homolytic scission of (8R)-hydroperoxylinoleic acid | Aspergillus fumigatus |
| Q890L | site-directed mutagenesis, the mutant shows almost abolished 5,8-linoleate diol synthase activity | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Gaeumannomyces graminis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleate + O2 | - |
Aspergillus fumigatus | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? |  |
| linoleate + O2 | - |
Gaeumannomyces graminis | (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate | - |
? | |
| additional information | the bifunctional enzyme forms (7S,8S)-7,8-dihydroxylinoleic acid from (8R)-hydroperoxylinoleic acid by intramolecular oxygen transfer. Linoleate diol synthases are fungal dioxygenase-cytochrome P450 fusion enzymes. P450 hydroxylases usually contain an acid-alcohol pair in the I-helices for the heterolytic scission of O2 and formation of compound I, i.e. Por+-Fe(IV)=O, and water. The function of the acid-alcohol pair appears to be replaced by a different amide residue, Asn938 of 7,8-LDS, for heterolysis of (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate to generate compound I | Gaeumannomyces graminis | ? | - |
? | |
| additional information | the bifunctional enzyme forms 5S,8S-dihydroxylinoleic acid from (8R)-hydroperoxylinoleic acid by intramolecular oxygen transfer. Linoleate diol synthases are fungal dioxygenase-cytochrome P450 fusion enzymes. P450 hydroxylases usually contain an acid-alcohol pair in the I-helices for the heterolytic scission of O2 and formation of compound I, i.e. Por+-Fe(IV)=O, and water. The function of the acid-alcohol pair appears to be replaced by a different amide residue, Gln890 of 5,8-LDS, for heterolysis of (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate to generate compound I | Aspergillus fumigatus | ? | - |
? | |
| additional information | the enzyme forms (5S,8R)-5,8-dihydroxylinoleic acid from (8R)-hydroperoxylinoleic acid by intramolecular oxygen transfer. Linoleate diol synthases are fungal dioxygenase-cytochrome P450 fusion enzymes | Aspergillus fumigatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | homology model of 5,8-LDS, overview | Aspergillus fumigatus |
| More | homology model of 7,8-LDS, overview | Gaeumannomyces graminis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5,8-LDS | - |
Aspergillus fumigatus |
| 5,8-linoleate diol synthase | - |
Aspergillus fumigatus |
| 7,8-LDS | - |
Gaeumannomyces graminis |
| 7,8-linoleate diol synthase | - |
Gaeumannomyces graminis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | homology model of 5,8-LDS, overview | Aspergillus fumigatus |
| additional information | homology model of 7,8-LDS, overview | Gaeumannomyces graminis |
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