Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.60 extracted from

  • Garscha, U.; Oliw, E.H.
    Leucine/valine residues direct oxygenation of linoleic acid by (10R)- and (8R)-dioxygenases: expression and site-directed mutagenesis oF (10R)-dioxygenase with epoxyalcohol synthase activity (2009), J. Biol. Chem., 284, 13755-13765.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.60

Cloned(Commentary)

Cloned (Comment) Organism
expression in SF21 cells Gaeumannomyces graminis

Protein Variants

Protein Variants Comment Organism
E384A mutant forms only traces of (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate Gaeumannomyces graminis
I375A mutant has virtually no dioxygenase activity Gaeumannomyces graminis
L334V inactive mutant enzyme Gaeumannomyces graminis
V330L the wild-type enzyme forms 98% (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate and 2% (8E,10R,12Z)-10-hydroperoxy-9,12-octadecadienoate. The V330L mutation augments the formation of (8E,10R,12Z)-10-hydroperoxy-8,12-octadecadienoate 3fold Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Gaeumannomyces graminis i.e. (9Z,12Z)-octadeca-9,12-dienoate (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2 i.e. (9Z,12Z)-octadeca-9,12-dienoate Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 i.e. (9Z,12Z)-octadeca-9,12-dienoate Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate the wild-type enzyme forms 98% (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate and 2% (10R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate. The V330L mutation augments the formation of (10R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate 3fold ?

Synonyms

Synonyms Comment Organism
7,8-LDS bifunctional enzyme Gaeumannomyces graminis
7,8-linoleate diol synthase bifunctional enzyme Gaeumannomyces graminis