Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.60 extracted from

  • Su, C.; Oliw, E.H.
    Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as anovel hemoprotein (1996), J. Biol. Chem., 271, 14112-14118.
    View publication on PubMed
Search for more literature for 1.13.11.60

General Stability

General Stability Organism
repeated freezing and thawing, particularly of dilute enzyme solutions, inactivates the enzyme Gaeumannomyces graminis
the enzyme is quite unstable at the early steps of purification, but active fractions from anion-exchange chromatography can be kept for 1 week on ice with only a small loss of enzyme activity Gaeumannomyces graminis

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1 mM KCN. CO does not inhibit the enzyme Gaeumannomyces graminis
N-[(E)-3-(3-phenoxyphenyl)prop-2-enyl]acetohydroxamic acid i.e. BW A4C, more than 90% inhibition at 0.01 mM, about 50% inhibition at 0.001 mM Gaeumannomyces graminis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 linoleate pH 7.4 25°C Gaeumannomyces graminis
0.03
-
 O2 pH 7.4 25°C Gaeumannomyces graminis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 4 * 130000, gel filtration Gaeumannomyces graminis
520000
-
 gel filtration Gaeumannomyces graminis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleate + O2 Gaeumannomyces graminis
-
 (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
urea the enzyme can be incubated in 4 M urea for 1 h, followed by desalting, with only partial loss of enzyme activity Gaeumannomyces graminis

Organism

Organism UniProt Comment Textmining
Gaeumannomyces graminis Q9UUS2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gaeumannomyces graminis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Gaeumannomyces graminis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 pH 7.4, 0°C Gaeumannomyces graminis

Storage Stability

Storage Stability Organism
-80°C, stable Gaeumannomyces graminis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleate + O2
-
 Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?
linoleate + O2 H2O2 (up to 9 mM) does not support enzyme activity under anaerobic conditions Gaeumannomyces graminis (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 130000, gel filtration Gaeumannomyces graminis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15
-
 linoleate pH 7.4, 0°C Gaeumannomyces graminis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Gaeumannomyces graminis

Cofactor

Cofactor Comment Organism Structure
heme the enzyme contains at least 2 mol of heme/mol of enzyme Gaeumannomyces graminis

pI Value

Organism Comment pI Value Maximum pI Value
Gaeumannomyces graminis chromatofocusing
-
 5.2