Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | - |
Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
3-hydroxyanthranilic acid | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | the most effective salt, the optimal concentration is 0.035 M | Bos taurus | |
Fe2+ | solvent, acid and heat function to modify the protein configuration so that ferrous ions can be bound to the enzyme to generate the most active form | Bos taurus | |
HCl | during purification of enzyme treatment with acid was used | Bos taurus | |
MgCl2 | - |
Bos taurus | |
NaCl | - |
Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | drastic change in the Km of the enzyme in the presence of dimethylglutarate buffer | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
reaches a maximum after 10 min at acid pH | Bos taurus |
Storage Stability | Organism |
---|---|
-15°C, in 0.01 M Tris buffer, pH 7.0, 4 days, no loss of activity | Bos taurus |
4°C, overnight, about 75% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyanthranilate + O2 | - |
Bos taurus | 2-amino-3-carboxymuconate semialdehyde | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
activation by heating at 55°C for 5 min | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at pH 3.4: the enzyme is in a form which can bind substrate but is enzymatically inactive, at pH 6.5: active form of enzyme | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
4°C, half-life: 3 days | Bos taurus |