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Literature summary for 1.13.11.58 extracted from
- Microbial synthesis of linoleate 9S-lipoxygenase derived plant C18 oxylipins from C18 polyunsaturated fatty acids (2019), J. Agric. Food Chem., 67, 3209-3219 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Myxococcus xanthus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
gamma-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus |  |
| 0.081 | - |
alpha-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
| 0.33 | - |
linoleic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Myxococcus xanthus | - |
- |
- |
| Myxococcus xanthus DK1622 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-Trap affinity chromatography | Myxococcus xanthus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid + O2 | - |
Myxococcus xanthus | 12,13-epoxy-14-hydroxy-cis,cis-9,15-octadecadienoic acid | - |
? | |
| 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid + O2 | - |
Myxococcus xanthus DK1622 | 12,13-epoxy-14-hydroxy-cis,cis-9,15-octadecadienoic acid | - |
? | |
| 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid + O2 | - |
Myxococcus xanthus | 9,10-epoxy-11-hydroxy-cis,cis-6,12-octadecadienoic acid | - |
? | |
| 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid + O2 | - |
Myxococcus xanthus DK1622 | 9,10-epoxy-11-hydroxy-cis,cis-6,12-octadecadienoic acid | - |
? | |
| 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid + O2 | - |
Myxococcus xanthus | 9,10-epoxy-11-hydroxy-cis-12-octadecenoic acid | - |
? | |
| 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid + O2 | - |
Myxococcus xanthus DK1622 | 9,10-epoxy-11-hydroxy-cis-12-octadecenoic acid | - |
? | |
| alpha-linolenic acid + O2 | - |
Myxococcus xanthus | 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid | - |
? | |
| alpha-linolenic acid + O2 | - |
Myxococcus xanthus DK1622 | 12-hydroperoxy-cis,trans,cis-9,13,15-octadecatrienoic acid | - |
? | |
| gamma-linoleic acid + O2 | - |
Myxococcus xanthus | 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid | - |
? | |
| gamma-linoleic acid + O2 | - |
Myxococcus xanthus DK1622 | 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid | - |
? | |
| gamma-linolenic acid + O2 | - |
Myxococcus xanthus | 9 9-hydroperoxy-cis,trans,cis-6,10,12-octadecatrienoic acid | - |
? | |
| linoleic acid + O2 | - |
Myxococcus xanthus | 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid | - |
? | |
| linolenic acid + O2 | - |
Myxococcus xanthus | 9-hydroperoxy-trans,cis-10,12-octadecadienoic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76000, His-tagged enzyme, SDS-PAGE | Myxococcus xanthus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| linoleate 9S-lipoxygenase | - |
Myxococcus xanthus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
gamma-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
| 1.27 | - |
alpha-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
| 7.95 | - |
linoleic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.05 | - |
gamma-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
| 15.7 | - |
alpha-linolenic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
| 24.1 | - |
linoleic acid | at pH 8.5 and 35°C | Myxococcus xanthus | |
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