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Literature summary for 1.13.11.54 extracted from

  • Dai, Y.; Pochapsky, T.C.; Abeles, R.H.
    Mechanistic studies of two dioxygenases in the methionine salvage pathway of Klebsiella pneumoniae (2001), Biochemistry, 40, 6379-6387.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
O2
-
Klebsiella pneumoniae
0.052
-
1,2-dihydroxyhex-1-en-3-one
-
Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe enzyme contains 1 atom of Fe Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
1 * 20000, SDS-PAGE Klebsiella pneumoniae
20240
-
mass spectrometry Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Klebsiella pneumoniae
-
3-(methylthio)propanoate + formate + CO
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2
-
Klebsiella pneumoniae 3-(methylthio)propanoate + formate + CO
-
?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 ordered-sequential mechanism Klebsiella pneumoniae 4-(methylthio)-2-oxobutanoate + formate
-
?
1,2-dihydroxyhex-1-en-3-one + O2 incorporation of O2 into C1 and C2 of 1,2-dihydroxy-3-keto-1-hexene Klebsiella pneumoniae 2-oxopentanoate + formate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 20000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
ARD'
-
Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
260
-
O2
-
Klebsiella pneumoniae
260
-
1,2-dihydroxyhex-1-en-3-one
-
Klebsiella pneumoniae