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Literature summary for 1.13.11.53 extracted from

  • Dai, Y.; Wensink, P.C.; Abeles, R.H.
    One protein, two enzymes (1999), J. Biol. Chem., 274, 1193-1195.
    View publication on PubMed
Search for more literature for 1.13.11.53

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
500
-
 O2
-
 Klebsiella pneumoniae
500
-
 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one
-
 Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ apoenzyme is catalytically inactive. Addition of Ni2+ or Co2+ yields activity. Production in intact Escherichia coli of E-2' depends on the availability of the Fe2+. Enzyme contains 1.1 Ni2+ per enzyme molecule Klebsiella pneumoniae
Ni2+ apoenzyme is catalytically inactive. Addition of Ni2+ or Co2+ yields activity. Production in intact Escherichia coli of E-2' depends on the availability of the Fe2+. Enzyme contains 1.1 Ni2+ per enzyme molecule Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20250
-
 mass spectrometry Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Klebsiella pneumoniae enzyme of the methionine salvage pathway 3-(methylthio)propanoate + formate + CO
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 enzyme of the methionine salvage pathway Klebsiella pneumoniae 3-(methylthio)propanoate + formate + CO
-
 ?
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 Ni2+ or Co2+ bound to the enzyme protein. If Fe2+ is bound instead of Ni2+ reaction catalyzed by EC 1.13.11.54 occurs instead Klebsiella pneumoniae 3-(methylthio)propanoate + formate + CO
-
 ?