Literature summary for 1.13.11.52 extracted from

Fukumura, E.; Sugimoto, H.; Misumi, Y.; Ogura, T.; Shiro, Y.
Cooperative binding of L-trp to human tryptophan 2,3-dioxygenase: resonance Raman spectroscopic analysis (2009), J. Biochem., 145, 505-515.

Search for more literature for 1.13.11.52

Cloned(Commentary)

Cloned (Comment)	Organism
hexa-histidine tag, expressed in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H76A	active site, tryptophan binding	Homo sapiens
Y42F	active site, tryptophan binding	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0825	-	L-tryptophan	pH 8.0, normal atmospheric conditions	Homo sapiens
0.18	-	L-tryptophan	pH 7.0, normal atmospheric conditions	Homo sapiens
0.488	-	L-tryptophan	H76A mutant enzyme, pH 8.0, normal atmospheric conditions	Homo sapiens
1.29	-	L-tryptophan	Y42F mutant enzyme, pH 8.0, normal atmospheric conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + O2	-	Homo sapiens	N-formylkynurenine	-	?

Subunits

Subunits	Comment	Organism
tetramer	gel filtration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
tryptophan 2,3-dioxygenase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.04	-	L-tryptophan	H76A mutant enzyme, pH 8.0, normal atmospheric conditions	Homo sapiens
0.6	-	L-tryptophan	pH 7.0, normal atmospheric conditions	Homo sapiens
3.04	-	L-tryptophan	Y42F mutant enzyme, pH 8.0, normal atmospheric conditions	Homo sapiens
6.12	-	L-tryptophan	pH 8.0, normal atmospheric conditions	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)