Literature summary for 1.13.11.52 extracted from

Sheoran, A.; King, A.; Velasco, A.; Pero, J.M.; Garneau-Tsodikova, S.
Characterization of TioF, a tryptophan 2,3-dioxygenase involved in 3-hydroxyquinaldic acid formation during thiocoraline biosynthesis (2008), Mol. Biosyst., 4, 622-628.

Search for more literature for 1.13.11.52

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.44	-	D-tryptophan	-	Micromonospora sp.
0.65	-	L-tryptophan	-	Micromonospora sp.
2.56	-	serotonin	-	Micromonospora sp.
30.27	-	indole	-	Micromonospora sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan + O2	Micromonospora sp.	first step during biosynthesis of 3-hydroxyquinaldic acid	N-formyl-L-kynurenine	-	ir

Organism

Organism	UniProt	Comment	Textmining
Micromonospora sp.	-	recombinantly expressed in Escherichia coli as a N-terminally His6-tagged protein	-
Micromonospora sp. ML1	-	recombinantly expressed in Escherichia coli as a N-terminally His6-tagged protein	-

Purification (Commentary)

Purification (Comment)	Organism
-	Micromonospora sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-tryptophan + O2	-	Micromonospora sp.	N-formyl-D-kynurenine	-	ir
D-tryptophan + O2	-	Micromonospora sp. ML1	N-formyl-D-kynurenine	-	ir
indole + O2	-	Micromonospora sp.	?	-	?
indole + O2	-	Micromonospora sp. ML1	?	-	?
L-tryptophan + O2	-	Micromonospora sp.	N-formyl-L-kynurenine	-	ir
L-tryptophan + O2	first step during biosynthesis of 3-hydroxyquinaldic acid	Micromonospora sp.	N-formyl-L-kynurenine	-	ir
L-tryptophan + O2	-	Micromonospora sp. ML1	N-formyl-L-kynurenine	-	ir
additional information	TioF is completely inactive towards tryptamine, 5-hydroxy-L-Trp, and indole propionic acid	Micromonospora sp.	?	-	?
additional information	TioF is completely inactive towards tryptamine, 5-hydroxy-L-Trp, and indole propionic acid	Micromonospora sp. ML1	?	-	?
serotonin + O2	-	Micromonospora sp.	?	-	?
serotonin + O2	-	Micromonospora sp. ML1	?	-	?

Synonyms

Synonyms	Comment	Organism
TioF	-	Micromonospora sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0068	-	D-tryptophan	-	Micromonospora sp.
0.028	-	serotonin	-	Micromonospora sp.
0.045	-	L-tryptophan	-	Micromonospora sp.
0.248	-	indole	-	Micromonospora sp.

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Release 2023.1 (January 2023)