Application | Comment | Organism |
---|---|---|
medicine | first and rate-limiting enzyme in tryptophan metabolism, plays a role in pathogenesis of many diseases | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli E538, formation of inclusion bodies at 37°C with reduced formation, at 30°C, expressed with His-Tag | Homo sapiens |
expression of His-tagged enzyme in Escherichia coli, optimised expression | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | first and rate-limiting enzyme in tryptophan metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-agarose and gel filtration (Superdex 75) | Homo sapiens |
the use of ALA, the biosynthetic precursor of protoporphyrin IX, coupled with metal-affinity chromatography and size exclusion chromatography produces 6His-IDP with a protein to heme ratio of 1:2.2 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.67 | - |
- |
Homo sapiens |
2.67 | - |
kynurenine, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.2 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 60 min | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | first and rate-limiting enzyme in tryptophan metabolism | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Homo sapiens |
indoleamine 2,3-dioxygenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | hemoprotein. The use of ALA, the biosynthetic precursor of protoporphyrin IX, coupled with metal-affinity chromatography and size exclusion chromatography produces 6His-IDP with a protein to heme ratio of 1:2.2 | Homo sapiens |