Activating Compound | Comment | Organism | Structure |
---|---|---|---|
gamma-interferon | - |
Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | enzyme catalyzes the first and rate-limiting step in kynurenine pathway | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D274A | mutant without enzyme activity, may be distal ligand or essential in maintaining the conformation of the heme pocket | Homo sapiens |
H16A | does not act as proximal ligand | Homo sapiens |
H303A | does not act as proximal ligand | Homo sapiens |
H346A | mutant without enzyme activity, His346 is essential for heme binding | Homo sapiens |
K352A | mutant with diminished heme binding ability | Homo sapiens |
V109A | mutant maintained heme binding ability | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
D-tryptophan | H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0024 | - |
D-tryptophan | H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0044 | - |
D-tryptophan | K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.005 | - |
D-tryptophan | wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0053 | - |
D-tryptophan | V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0056 | - |
L-tryptophan | H16A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0089 | - |
L-tryptophan | V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0094 | - |
L-tryptophan | H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.02 | - |
L-tryptophan | wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.0201 | - |
L-tryptophan | K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.1 | - |
5-hydroxy-L-tryptophan | H303A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.1 | - |
5-hydroxy-L-tryptophan | V109A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.14 | - |
5-hydroxy-L-tryptophan | H16A, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.17 | - |
5-hydroxy-L-tryptophan | K352A mutant, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens | |
0.4 | - |
5-hydroxy-L-tryptophan | wild type, 50 mM potassium phosphate buffer (pH 6.5), 20 mM ascorbic acid, 0.3 mg/ml catalase, 0.01 mM methylene blue, 0.4 mM tryptophan, 37°C, 10-60 min | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-L-tryptophan + O2 | - |
Homo sapiens | N-formyl-5-hydroxy-L-kynurenine | - |
? | |
D-tryptophan + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Homo sapiens | N-formyl-L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Homo sapiens |