Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.52 extracted from

  • Shimizu, T.; Nomiyama, S.; Hirata, F.; Hayaishi, O.
    Indoleamine 2,3-dioxygenase. Purification and some properties (1978), J. Biol. Chem., 253, 4700-4706.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
H2O2 inactivated by H2O formed by ascorbic acid in presence of methylen blue Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
5-hydroxy-L-tryptophan 24°C Oryctolagus cuniculus
0.045
-
L-Trp 24°C Oryctolagus cuniculus
0.075
-
tryptamine 24°C Oryctolagus cuniculus
0.1
-
serotonin 24°C Oryctolagus cuniculus
0.4
-
D-Trp 24°C Oryctolagus cuniculus
0.6
-
5-hydroxy-D-tryptophan 24°C Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 40000, SDS-PAGE Oryctolagus cuniculus
41000
-
1 * 41000, sedimentation velocity experiments in presence of 6 M guanidine HCl Oryctolagus cuniculus
42000
-
sedimentation velocity experiments Oryctolagus cuniculus
54000
-
gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 5.4% carbohydrate Oryctolagus cuniculus
glycoprotein 1.2 galactose, 2.6 mannose, 5.2 N-acetylglucosamine and 0.8 sialic acid residues per mol of enzyme Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
small intestine
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.85
-
-
Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-70°C, stable for at least 2 months Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-5-hydroxytryptophan + O2
-
Oryctolagus cuniculus ?
-
?
D-Trp + O2
-
Oryctolagus cuniculus N-formyl-D-kynurenine
-
?
L-5-hydroxytryptophan + O2
-
Oryctolagus cuniculus ?
-
?
L-Trp + O2
-
Oryctolagus cuniculus L-formylkynurenine
-
?
additional information in the presence of guaiacol or potassium iodide the enzyme degrades H2O2 Oryctolagus cuniculus ?
-
?
serotonin + O2
-
Oryctolagus cuniculus ?
-
?
tryptamine + O2
-
Oryctolagus cuniculus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Oryctolagus cuniculus
monomer 1 * 41000, sedimentation velocity experiments in presence of 6 M guanidine HCl Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 6.0, 5 min, 43% loss of dioxygenase activity on L-Trp and D-Trp, 55% loss ofter 10 min, 70% loss ofter 20 min Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00283
-
serotonin 24°C Oryctolagus cuniculus
0.00483
-
tryptamine 24°C Oryctolagus cuniculus
0.00967
-
5-hydroxy-D-tryptophan 24°C Oryctolagus cuniculus
0.0433
-
5-hydroxy-L-tryptophan 24°C Oryctolagus cuniculus
1.18
-
D-Trp 24°C Oryctolagus cuniculus
1.65
-
L-Trp
-
Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
4°C, 6 h, 52% loss of activity Oryctolagus cuniculus
5
-
4°C, 6 h, 7% loss of activity Oryctolagus cuniculus
6
-
4°C, no loss of activity after 6 h Oryctolagus cuniculus
7
-
4°C, 6 h, 15% loss of activity Oryctolagus cuniculus
8
-
4°C, 6 h, 55% loss of activity Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
heme 0.8 mol of protoheme IX per mol of enzyme Oryctolagus cuniculus