Literature summary for 1.13.11.52 extracted from

Feigelson, P.; Brady, F.O.
Heme-containing dioxygenases (1974), Mol. Mech. Oxygen Activ. (Hayaishi, O., ed.) Academic Press, New York, , 87-133.

No PubMed abstract available

Search for more literature for 1.13.11.52

Inhibitors

Inhibitors	Comment	Organism	Structure
DL-4-fluorotryptophan	-	Delftia acidovorans
DL-4-fluorotryptophan	-	Rattus norvegicus
DL-4-methyltryptophan	-	Delftia acidovorans
DL-4-methyltryptophan	-	Rattus norvegicus
DL-5-fluorotryptophan	-	Delftia acidovorans
DL-5-fluorotryptophan	-	Rattus norvegicus
DL-5-Methyltryptophan	-	Delftia acidovorans
DL-5-Methyltryptophan	-	Rattus norvegicus
indole	-	Delftia acidovorans
indole	-	Rattus norvegicus
Indolepropionic acid	-	Delftia acidovorans
Indolepropionic acid	-	Rattus norvegicus
L-5-hydroxytryptophan	-	Delftia acidovorans
L-5-hydroxytryptophan	-	Rattus norvegicus
additional information	no inhibition by D-5-hydroxytryptophan	Delftia acidovorans
additional information	no inhibition by D-5-hydroxytryptophan	Rattus norvegicus
tryptamine	-	Delftia acidovorans
tryptamine	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.037	-	O2	pH 8.0	Rattus norvegicus
0.038	-	O2	pH 7.0	Delftia acidovorans
0.25	-	L-Trp	pH 8.0	Rattus norvegicus
0.3	-	L-Trp	pH 7.0	Delftia acidovorans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	enzyme contains 2 copper moieties	Rattus norvegicus
copper	enzyme contains 2 copper moieties	Delftia acidovorans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	4 * 31000, SDS-PAGE	Delftia acidovorans
43000	-	alpha2beta2, 2 * 43000 + 2 * 43000, SDS-PAGE	Rattus norvegicus
122000	-	gel filtration, sucrose density gradient centrifugation, equilibrium sedimentation	Delftia acidovorans
167000	-	gel filtration, sucrose density gradient centrifugation, equilibrium sedimentation	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-Trp + O2	Rattus norvegicus	regulation of enzyme activity	L-formylkynurenine	-	?
L-Trp + O2	Delftia acidovorans	regulation of enzyme activity	L-formylkynurenine	-	?

Organism

Organism	UniProt	Comment	Textmining
Delftia acidovorans	-	-	-
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Delftia acidovorans

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.5	-	-	Rattus norvegicus
17	-	-	Delftia acidovorans

Storage Stability

Storage Stability	Organism
pH 7.0, anaerobically stored in 0.1 M potassium phosphate buffer containing L-Trp, quite stable	Rattus norvegicus
pH 7.0, anaerobically stored in 0.1 M potassium phosphate buffer containing L-Trp, quite stable	Delftia acidovorans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DL-6-fluorotryptophan + O2	-	Rattus norvegicus	?	-	?
DL-6-fluorotryptophan + O2	-	Delftia acidovorans	?	-	?
DL-6-methyltryptophan	-	Rattus norvegicus	?	-	?
L-Trp + O2	-	Rattus norvegicus	L-formylkynurenine	-	?
L-Trp + O2	-	Delftia acidovorans	L-formylkynurenine	-	?
L-Trp + O2	regulation of enzyme activity	Rattus norvegicus	L-formylkynurenine	-	?
L-Trp + O2	regulation of enzyme activity	Delftia acidovorans	L-formylkynurenine	-	?
additional information	no activity with D-Trp	Rattus norvegicus	additional information	-	?
additional information	no activity with D-Trp	Delftia acidovorans	additional information	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 31000, SDS-PAGE	Delftia acidovorans
tetramer	alpha2beta2, 2 * 43000 + 2 * 43000, SDS-PAGE	Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	2070 per min, formylkynurenine formed	Delftia acidovorans
additional information	-	additional information	420 per min, formylkynurenine formed	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
heme	enzyme contains two heme moieties	Rattus norvegicus
heme	enzyme contains two heme moieties	Delftia acidovorans

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.001	-	L-5-hydroxytryptophan	-	Delftia acidovorans
0.01	-	L-5-hydroxytryptophan	-	Rattus norvegicus
0.08	-	Indolepropionic acid	-	Delftia acidovorans
0.1	-	tryptamine	-	Delftia acidovorans
0.12	-	DL-5-fluorotryptophan	-	Rattus norvegicus
0.14	-	indole	-	Rattus norvegicus
0.19	-	DL-5-Methyltryptophan	-	Rattus norvegicus
0.21	-	Indolepropionic acid	-	Delftia acidovorans
0.28	-	DL-4-methyltryptophan	-	Rattus norvegicus
0.33	-	DL-4-fluorotryptophan	-	Rattus norvegicus
0.39	-	DL-5-fluorotryptophan	-	Delftia acidovorans
0.55	-	DL-5-Methyltryptophan	-	Delftia acidovorans
0.67	-	DL-4-methyltryptophan	-	Delftia acidovorans
1	-	DL-4-fluorotryptophan	-	Delftia acidovorans
1.8	-	tryptamine	-	Delftia acidovorans
2.7	-	indole	-	Delftia acidovorans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)