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show all sequences of 1.13.11.50

Kinetic and CD/MCD spectroscopic studies of the atypical, three-His-ligated, non-heme Fe2+ center in diketone dioxygenase: the role of hydrophilic outer shell residues in catalysis

Straganz, G.D.; Diebold, A.R.; Egger, S.; Nidetzky, B.; Solomon, E.I.; Biochemistry 49, 996-1004 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E98A
site-direted mutagenesis
Acinetobacter johnsonii
E98Q
site-direted mutagenesis
Acinetobacter johnsonii
R80A
site-direted mutagenesis
Acinetobacter johnsonii
R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
T107A
site-direted mutagenesis
Acinetobacter johnsonii
Y70A
site-direted mutagenesis
Acinetobacter johnsonii
Y70A/R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
Y70F
site-direted mutagenesis
Acinetobacter johnsonii
Y70F/R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview
Acinetobacter johnsonii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
Dke1 contains an atypical, three-histidine-ligated, mononuclear non-heme Fe2+ center, spectroscopic analysis, overview. Stabilizing effect of Glu98 on the 6C geometry of the metal center, priming it for substrate ligation. Also Thr107 stabilizes the Fe(II) cofactor
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pentane-2,4-dione + O2
Acinetobacter johnsonii
-
acetate + 2-oxopropanal
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter johnsonii
Q8GNT2
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1,1-trifluoro-pentane-2,4-dione + O2
-
711267
Acinetobacter johnsonii
?
-
-
-
?
additional information
residues Arg80 and Tyr70 promote O2 reduction
711267
Acinetobacter johnsonii
?
-
-
-
-
pentane-2,4-dione + O2
-
711267
Acinetobacter johnsonii
acetate + 2-oxopropanal
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acinetobacter johnsonii
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.007
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A
Acinetobacter johnsonii
0.033
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A
Acinetobacter johnsonii
0.05
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant E98Q
Acinetobacter johnsonii
0.074
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant E98A
Acinetobacter johnsonii
0.098
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant R80A/E98A
Acinetobacter johnsonii
0.1
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant T107A
Acinetobacter johnsonii
0.126
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant R80A
Acinetobacter johnsonii
0.13
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70A
Acinetobacter johnsonii
1.27
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70F
Acinetobacter johnsonii
6.6
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant wild-type enzyme
Acinetobacter johnsonii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Acinetobacter johnsonii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10.5
-
Acinetobacter johnsonii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E98A
site-direted mutagenesis
Acinetobacter johnsonii
E98Q
site-direted mutagenesis
Acinetobacter johnsonii
R80A
site-direted mutagenesis
Acinetobacter johnsonii
R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
T107A
site-direted mutagenesis
Acinetobacter johnsonii
Y70A
site-direted mutagenesis
Acinetobacter johnsonii
Y70A/R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
Y70F
site-direted mutagenesis
Acinetobacter johnsonii
Y70F/R80A/E98A
site-direted mutagenesis
Acinetobacter johnsonii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview
Acinetobacter johnsonii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
Dke1 contains an atypical, three-histidine-ligated, mononuclear non-heme Fe2+ center, spectroscopic analysis, overview. Stabilizing effect of Glu98 on the 6C geometry of the metal center, priming it for substrate ligation. Also Thr107 stabilizes the Fe(II) cofactor
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pentane-2,4-dione + O2
Acinetobacter johnsonii
-
acetate + 2-oxopropanal
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,1,1-trifluoro-pentane-2,4-dione + O2
-
711267
Acinetobacter johnsonii
?
-
-
-
?
additional information
residues Arg80 and Tyr70 promote O2 reduction
711267
Acinetobacter johnsonii
?
-
-
-
-
pentane-2,4-dione + O2
-
711267
Acinetobacter johnsonii
acetate + 2-oxopropanal
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Acinetobacter johnsonii
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.007
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70A/R80A/E98A
Acinetobacter johnsonii
0.033
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70F/R80A/E98A
Acinetobacter johnsonii
0.05
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant E98Q
Acinetobacter johnsonii
0.074
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant E98A
Acinetobacter johnsonii
0.098
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant R80A/E98A
Acinetobacter johnsonii
0.1
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant T107A
Acinetobacter johnsonii
0.126
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant R80A
Acinetobacter johnsonii
0.13
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70A
Acinetobacter johnsonii
1.27
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant mutant Y70F
Acinetobacter johnsonii
6.6
-
1,1,1-trifluoro-pentane-2,4-dione
pH 7.5, 25°C, recombinant wild-type enzyme
Acinetobacter johnsonii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Acinetobacter johnsonii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10.5
-
Acinetobacter johnsonii
Other publictions for EC 1.13.11.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742322
Brkic
-
Insight of the iron binding a ...
Acinetobacter johnsonii
Croat. Chem. Acta
88
297-306
2015
-
-
-
1
3
-
-
-
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1
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1
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725553
Brkic
Dke1--structure, dynamics, and ...
Acinetobacter johnsonii
J. Biol. Inorg. Chem.
17
801-815
2012
-
-
1
-
4
-
-
1
-
2
-
1
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1
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1
-
-
-
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2
1
1
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10
1
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1
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4
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-
1
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2
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1
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1
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-
-
2
1
1
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-
10
1
-
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2
2
-
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-
725198
Diebold
Spectroscopic and computationa ...
Acinetobacter johnsonii
J. Am. Chem. Soc.
133
15979-15991
2011
-
-
-
-
-
-
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1
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2
-
1
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3
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1
1
-
-
-
711258
Diebold
The three-his triad in Dke1: c ...
Acinetobacter johnsonii
Biochemistry
49
6945-6952
2010
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2
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1
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1
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1
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2
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-
-
-
-
-
-
-
-
711267
Straganz
Kinetic and CD/MCD spectroscop ...
Acinetobacter johnsonii
Biochemistry
49
996-1004
2010
-
-
-
-
9
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
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3
-
1
-
-
10
1
1
-
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-
9
-
-
-
-
1
-
1
-
1
-
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-
-
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-
-
3
-
1
-
-
10
1
1
-
-
-
-
-
-
-
-
696107
Leitgeb
Biochemical characterization a ...
Acinetobacter johnsonii
Biochem. J.
418
403-411
2009
-
-
1
-
8
-
6
-
-
6
1
1
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
6
-
-
-
6
1
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675466
Straganz
-
Exploring the cupin-type metal ...
Acinetobacter johnsonii
J. Mol. Catal. B
39
171-178
2006
-
-
1
-
1
-
-
2
-
1
1
-
-
1
-
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1
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2
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3
1
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1
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1
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2
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1
1
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1
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2
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3
1
-
-
-
-
-
-
-
-
-
657614
Hofer
Fast determination of operatio ...
Acinetobacter johnsonii
Appl. Microbiol. Biotechnol.
1
1-12
2005
-
1
1
-
-
1
-
2
1
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
2
1
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-
1
-
-
-
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-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
657866
Grogan
Emergent mechanistic diversity ...
Acinetobacter johnsonii
Biochem. J.
388
721-730
2005
-
-
1
1
-
-
-
1
-
1
1
1
-
1
-
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1
-
1
-
-
6
1
-
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-
1
-
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-
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-
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1
-
1
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1
-
1
1
1
-
-
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-
1
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
671683
Straganz
-
Integrated approach for produc ...
Acinetobacter johnsonii
Biocatal. Biotransform.
23
261-269
2005
-
-
1
-
-
-
-
3
-
1
4
-
-
1
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1
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1
1
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3
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1
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3
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1
4
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1
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1
1
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3
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-
674150
Straganz
Reaction coordinate analysis f ...
Acinetobacter johnsonii
J. Am. Chem. Soc.
127
12306-12314
2005
-
-
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-
4
-
1
2
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3
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4
1
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4
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4
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1
2
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4
1
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4
-
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-
-
-
-
-
-
-
-
636327
Straganz
Acetylacetone-cleaving enzyme ...
Acinetobacter johnsonii
Biochem. J.
369
573-581
2003
-
-
1
-
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5
1
-
1
1
1
-
3
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1
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11
1
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1
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1
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5
-
1
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1
1
1
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1
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11
1
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-
1
-
-
-
-
-
-
-
-
636328
Straganz
A novel beta-diketone-cleaving ...
Acinetobacter johnsonii
Biochem. Biophys. Res. Commun.
297
232-236
2002
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-
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2
1
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1
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1
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5
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2
1
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1
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2
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5
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