BRENDA - Enzyme Database show
show all sequences of 1.13.11.50

The three-his triad in Dke1: comparisons to the classical facial triad

Diebold, A.R.; Neidig, M.L.; Moran, G.R.; Straganz, G.D.; Solomon, E.I.; Biochemistry 49, 6945-6952 (2010)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
the Fe(II) coordinating triad is composed of three His residues, geometric and electronic structure of the Fe(II) center, structure and function comparison with other dioxygenases containing a two histidines and a carboxylate coordinating the iron center in a facial triad, overview
Acinetobacter johnsonii
additional information
the enzyme is a mononuclear non-heme iron enzyme, overview
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetylacetone + O2
Acinetobacter johnsonii
-
acetate + 2-oxopentanal
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter johnsonii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetylacetone + O2
-
711258
Acinetobacter johnsonii
acetate + 2-oxopentanal
-
-
-
?
additional information
the enzyme is a mononuclear non-heme iron enzymes, Dke1 active site modelling, overview
711258
Acinetobacter johnsonii
?
-
-
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
the Fe(II) coordinating triad is composed of three His residues, geometric and electronic structure of the Fe(II) center, structure and function comparison with other dioxygenases containing a two histidines and a carboxylate coordinating the iron center in a facial triad, overview
Acinetobacter johnsonii
additional information
the enzyme is a mononuclear non-heme iron enzyme, overview
Acinetobacter johnsonii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetylacetone + O2
Acinetobacter johnsonii
-
acetate + 2-oxopentanal
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetylacetone + O2
-
711258
Acinetobacter johnsonii
acetate + 2-oxopentanal
-
-
-
?
additional information
the enzyme is a mononuclear non-heme iron enzymes, Dke1 active site modelling, overview
711258
Acinetobacter johnsonii
?
-
-
-
-
Other publictions for EC 1.13.11.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742322
Brkic
-
Insight of the iron binding a ...
Acinetobacter johnsonii
Croat. Chem. Acta
88
297-306
2015
-
-
-
1
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725553
Brkic
Dke1--structure, dynamics, and ...
Acinetobacter johnsonii
J. Biol. Inorg. Chem.
17
801-815
2012
-
-
1
-
4
-
-
1
-
2
-
1
-
1
-
-
1
-
-
-
-
-
2
1
1
-
-
10
1
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
1
-
2
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
10
1
-
-
-
-
2
2
-
-
-
725198
Diebold
Spectroscopic and computationa ...
Acinetobacter johnsonii
J. Am. Chem. Soc.
133
15979-15991
2011
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711258
Diebold
The three-his triad in Dke1: c ...
Acinetobacter johnsonii
Biochemistry
49
6945-6952
2010
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711267
Straganz
Kinetic and CD/MCD spectroscop ...
Acinetobacter johnsonii
Biochemistry
49
996-1004
2010
-
-
-
-
9
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
10
1
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
10
1
1
-
-
-
-
-
-
-
-
696107
Leitgeb
Biochemical characterization a ...
Acinetobacter johnsonii
Biochem. J.
418
403-411
2009
-
-
1
-
8
-
6
-
-
6
1
1
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
8
-
-
6
-
-
-
6
1
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675466
Straganz
-
Exploring the cupin-type metal ...
Acinetobacter johnsonii
J. Mol. Catal. B
39
171-178
2006
-
-
1
-
1
-
-
2
-
1
1
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
1
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
657614
Hofer
Fast determination of operatio ...
Acinetobacter johnsonii
Appl. Microbiol. Biotechnol.
1
1-12
2005
-
1
1
-
-
1
-
2
1
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
657866
Grogan
Emergent mechanistic diversity ...
Acinetobacter johnsonii
Biochem. J.
388
721-730
2005
-
-
1
1
-
-
-
1
-
1
1
1
-
1
-
-
-
1
-
1
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
1
1
1
-
-
-
-
-
1
-
-
6
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
671683
Straganz
-
Integrated approach for produc ...
Acinetobacter johnsonii
Biocatal. Biotransform.
23
261-269
2005
-
-
1
-
-
-
-
3
-
1
4
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
1
4
-
-
-
-
1
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
674150
Straganz
Reaction coordinate analysis f ...
Acinetobacter johnsonii
J. Am. Chem. Soc.
127
12306-12314
2005
-
-
-
-
-
-
-
4
-
1
2
-
-
3
-
-
-
-
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
2
-
-
-
-
-
-
-
-
-
4
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
636327
Straganz
Acetylacetone-cleaving enzyme ...
Acinetobacter johnsonii
Biochem. J.
369
573-581
2003
-
-
1
-
-
-
5
1
-
1
1
1
-
3
-
-
1
-
-
-
-
-
11
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
1
-
1
1
1
-
-
-
1
-
-
-
-
11
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
636328
Straganz
A novel beta-diketone-cleaving ...
Acinetobacter johnsonii
Biochem. Biophys. Res. Commun.
297
232-236
2002
-
-
-
-
-
-
-
-
-
2
1
-
-
1
-
-
1
-
-
-
2
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
2
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-