Application | Comment | Organism |
---|---|---|
environmental protection | biodegradation by the enzyme of the widely used industrial chemical acetylacetone, i.e. 2,4-pentanedione, which has toxic effects, in a membrane bioreactor, determination of operational stability of the enzyme in the reactor at different temperatures, simulations | Acinetobacter johnsonii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Acinetobacter johnsonii |
General Stability | Organism |
---|---|
soluble enzyme is poorly stable | Acinetobacter johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
Pentane-2,4-dione | pH 7.5, 25°C | Acinetobacter johnsonii | |
0.26 | - |
O2 | pH 7.5, 25°C | Acinetobacter johnsonii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Acinetobacter johnsonii | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + 2-oxopropanal | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetylacetone-grown cell | as sole carbon source | Acinetobacter johnsonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.34 | - |
recombinant enzyme, 35°C | Acinetobacter johnsonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetylacetone-cleaving enzyme | - |
Acinetobacter johnsonii |
Dke1 | - |
Acinetobacter johnsonii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Acinetobacter johnsonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
half-life 308 min in a membrane bioreactor, increase in stability from 5°C to 10°C | Acinetobacter johnsonii |
35 | - |
half-life 9 min in a membrane bioreactor | Acinetobacter johnsonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acinetobacter johnsonii |