BRENDA - Enzyme Database show
show all sequences of 1.13.11.5

Catalytic reaction mechanism of homogentisate dioxygenase: a hybrid DFT study

Borowski, T.; Georgiev, V.; Siegbahn, P.E.; J. Am. Chem. Soc. 127, 17303-17314 (2005)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q93099
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
homogentisate + O2
-
674157
Homo sapiens
4-maleylacetoacetate
-
-
-
ir
Subunits
Subunits
Commentary
Organism
homohexamer
or dimer of trimer, X-ray crystallography
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
homogentisate + O2
-
674157
Homo sapiens
4-maleylacetoacetate
-
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
homohexamer
or dimer of trimer, X-ray crystallography
Homo sapiens
Other publictions for EC 1.13.11.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742317
Bernini
Toward a generalized computat ...
Homo sapiens
Comput. Biol. Chem.
70
133-141
2017
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-
-
-
9
-
-
-
-
1
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1
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1
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10
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1
1
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9
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1
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1
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10
-
1
1
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-
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-
-
-
-
-
-
-
-
742328
Mori
Effects of homologous express ...
Phanerochaete sordida, Phanerochaete sordida YK-624
Curr. Microbiol.
73
512-518
2016
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1
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2
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6
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1
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1
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742389
Gonyar
Single amino acid substitutio ...
Burkholderia cepacia, Burkholderia cepacia K56-2
Environ. Microbiol. Rep.
7
180-187
2015
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1
1
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743037
Bernardini
Homogentisate 1,2 dioxygenase ...
Homo sapiens, Mus musculus
J. Inherit. Metab. Dis.
38
807-814
2015
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11
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2
2
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726390
Jeoung
Visualizing the substrate-, su ...
Pseudomonas putida, Pseudomonas putida KT 2240
Proc. Natl. Acad. Sci. USA
110
12625-12630
2013
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1
1
2
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1
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1
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2
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1
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1
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2
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1
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1
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3
3
-
2
2
725601
Laschi
Homogentisate 1,2 dioxygenase ...
Homo sapiens
J. Cell. Physiol.
227
3254-3257
2012
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5
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1
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1
1
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726475
Al-sbou
Novel mutations in the homogen ...
Homo sapiens
Rheumatol. Int.
32
1741-1746
2012
-
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1
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5
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2
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1
1
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726271
Mendez
The homogentisate and homoprot ...
Paraburkholderia xenovorans
PLoS ONE
6
e17583
2011
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1
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2
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1
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1
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1
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2
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2
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712561
Tinti
Evaluation of antioxidant drug ...
Homo sapiens
J. Cell. Physiol.
225
84-91
2010
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1
1
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712909
Toth
Familiar ochronotic arthropath ...
Homo sapiens
Joint Bone Spine
77
355-357
2010
-
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1
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1
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2
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1
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1
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1
1
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712929
Zhou
Meat quality and carcass trait ...
Bos taurus, Bos taurus Chinese red cattle
Meat Sci.
85
270-273
2010
-
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1
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1
1
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712935
Effelsberg
A metabolic cause of spinal de ...
Homo sapiens
Metab. Clin. Exp.
59
140-143
2010
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2
2
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712990
Zhou
Molecular cloning of the HGD g ...
Bos taurus
Mol. Biol. Rep.
37
603-611
2010
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1
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3
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697403
Grasko
A novel missense HGD gene muta ...
Homo sapiens
Clin. Chim. Acta
403
254-256
2009
-
1
1
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2
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1
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1
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1
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1
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1
1
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2
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1
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1
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699799
Munne-Bosch
Physiological and molecular re ...
Cistus creticus
J. Plant Physiol.
166
136-145
2009
1
-
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2
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706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
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2
2
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686266
Kang
Characterization of a homogent ...
Pseudomonas chlororaphis, Pseudomonas chlororaphis O6
Curr. Microbiol.
56
145-149
2008
-
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2
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4
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686802
Zhang
hmgA, transcriptionally activa ...
Streptomyces coelicolor
FEMS Microbiol. Lett.
280
219-225
2008
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686387
Yoon
Two-pronged survival strategy ...
Pseudomonas aeruginosa
EMBO J.
26
3662-3672
2007
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670622
Dixon
Enzymes of tyrosine catabolism ...
Arabidopsis thaliana
Plant Sci.
171
360-366
2006
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2
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677068
Kim
Analysis of aromatic catabolic ...
Pseudomonas putida, Pseudomonas putida KT 2440
Proteomics
6
1301-1318
2006
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657627
Gunsch
Identification of a homogentis ...
Exophiala lecanii-corni
Appl. Microbiol. Biotechnol.
68
405-411
2005
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1
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657859
Veldhuizen
Steady-state kinetics and inhi ...
Homo sapiens
Biochem. J.
386
305-314
2005
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1
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4
6
-
1
1
1
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2
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1
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1
1
3
1
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1
8
1
1
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1
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4
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6
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1
1
1
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1
1
3
1
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1
8
1
1
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670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
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2
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1
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-
-
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1
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674157
Borowski
Catalytic reaction mechanism o ...
Homo sapiens
J. Am. Chem. Soc.
127
17303-17314
2005
-
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1
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3
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1
1
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1
1
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439392
Titus
Crystal structure of human hom ...
Bos taurus, Homo sapiens, Mus musculus
Nat. Struct. Biol.
7
542-546
2000
-
2
-
1
2
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3
3
2
-
4
-
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1
1
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5
1
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5
1
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439387
Granadino
The human homogentisate 1,2-di ...
Homo sapiens
Genomics
43
115-122
1997
-
1
1
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5
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1
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439388
Schmidt
Cloning of the homogentisate 1 ...
Mus musculus
Mamm. Genome
8
168-171
1997
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1
1
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8
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1
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439389
Fernandez-Canon
Spectrophotometric determinati ...
Aspergillus nidulans
Anal. Biochem.
245
218-221
1997
2
1
-
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1
1
2
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3
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1
1
3
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439390
Hudecova
Purification of the homogentis ...
Bos taurus, Homo sapiens, Mus musculus, Oryctolagus cuniculus
Int. J. Biochem. Cell Biol.
27
1357-1363
1995
-
2
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2
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4
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4
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2
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3
1
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1
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439391
Fernandez-Canon
Molecular characterization of ...
Aspergillus nidulans, Aspergillus nidulans BiA1
J. Biol. Chem.
270
21199-21205
1995
1
-
1
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6
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1
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439393
Schmidt
Murine liver homogentisate 1,2 ...
Mus musculus
Eur. J. Biochem.
228
425-430
1995
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1
7
1
1
2
2
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1
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1
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2
1
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2
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1
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439386
Sugumaran
-
Affinity chromatography of hom ...
Aspergillus niger, Aspergillus niger UBC 814
FEMS Microbiol. Lett.
4
343-347
1978
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4
1
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4
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1
1
4
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1
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396333
Nozaki
-
Nonheme iron dioxygenase ...
Bos taurus, Pseudomonas fluorescens
Mol. Mech. Oxygen Activ. (Hayaishi, O., ed.) Academic Press, New York
135-165
1974
5
-
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1
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2
4
1
1
2
1
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2
1
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1
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1
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1
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439385
Durand
Enzymes of the homogentisate r ...
Agave toumeyana, Agrostemma githago, Daucus carota, Drosophyllum lusitanicum, Glycine max, Melilotus albus, Nicotiana sylvestris, Phaseolus vulgaris, Pimpinella anisum, Plectranthus scutellarioides, Ruta graveolens
FEBS Lett.
39
218-220
1974
-
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1
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-
11
-
11
-
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11
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11
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22
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11
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11
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22
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439380
Adachi
Purification and properties of ...
Pseudomonas fluorescens
Biochim. Biophys. Acta
118
88-97
1966
3
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-
1
-
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2
1
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1
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1
2
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1
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1
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1
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-
-
-
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439382
Knox
Homogentisate oxidase of liver ...
Rattus norvegicus
J. Biol. Chem.
216
479-487
1955
3
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-
11
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1
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-
439383
Crandall
Homogentisic acid oxidase. I. ...
Cavia porcellus, Oryctolagus cuniculus, Pigeon, Rattus norvegicus
J. Biol. Chem.
208
629-638
1954
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
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12
-
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-
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12
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
439381
Ravdin
The enzymatic conversion of ho ...
Rattus norvegicus
J. Biol. Chem.
189
137-149
1951
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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