Application | Comment | Organism |
---|---|---|
medicine | - |
Mus musculus |
medicine | inactivation of enzyme in kidney and liver causes the basic defect of alkaptonuria | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | human gene for alkaptonuria is mapped to chromosome 3q2 | Homo sapiens |
additional information | alkaptonuric mouse lacks enzyme activity and have recessive mutation aku, mapped to chromosome 16, inbred strain C57BLG/J is heterozygous for the aku mutation | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 0.001 mM, 56% inhibition | Oryctolagus cuniculus | |
HgCl2 | 20 mM, complete inhibition | Oryctolagus cuniculus | |
NaCl | 0.001 mM, 63% inhibition | Oryctolagus cuniculus | |
p-hydroxyphenylacetic acid | 80 mM, complete inhibition, substrate analogue | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
homogentisate | - |
Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Mus musculus |
450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Homo sapiens |
450000 | 480000 | sucrose density gradient centrifugation, can be degraded in two proteins of 200 and 230 kDa, probably due to inactivation by air | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | Mus musculus | - |
4-maleylacetoacetate | - |
? | |
homogentisate + O2 | Homo sapiens | enzyme catalyzes an intermediate step in the catabolism of tyrosine and phenylalanine | 4-maleylacetoacetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
normal and alkaptonuric mice, which completely lack HTO | - |
Oryctolagus cuniculus | - |
male rabbits | - |
Oxidation Stability | Organism |
---|---|
inactivation by air probably causes degradation of enzyme in two proteins | Oryctolagus cuniculus |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | enzyme is not glycosylated | Oryctolagus cuniculus |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Homo sapiens |
- |
Bos taurus |
54.1fold purification | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | liver from a 20 week old male embryo | Homo sapiens | - |
liver | - |
Mus musculus | - |
liver | - |
Bos taurus | - |
liver | - |
Oryctolagus cuniculus | - |
liver | embryonal liver | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mus musculus |
additional information | - |
- |
Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | - |
Mus musculus | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | - |
Homo sapiens | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | - |
Oryctolagus cuniculus | 4-maleylacetoacetate | - |
? | |
homogentisate + O2 | enzyme catalyzes an intermediate step in the catabolism of tyrosine and phenylalanine | Homo sapiens | 4-maleylacetoacetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 450000-480000, SDS-PAGE | Mus musculus |
monomer | 1 * 450000-480000, SDS-PAGE | Homo sapiens |
monomer | 1 * 450000-480000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Oryctolagus cuniculus |