Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required for maximal activity at pH 6 | Pseudomonas fluorescens | |
cysteine | activates | Pseudomonas fluorescens | |
glutathione | required for maximal activity at pH 6 and 5.4 | Pseudomonas fluorescens |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
General Stability | Organism |
---|---|
unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Pseudomonas fluorescens | |
p-chloromercuribenzoate | competitive inhibition with respect to Fe2+ but not to homogentisate | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
homogentisate | at pH 6 | Pseudomonas fluorescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | Fe2+ is required as cofactor | Pseudomonas fluorescens | |
additional information | other metallic ions, such as Fe3+, Co2+, Mn2+, Cu+ and Cu2+, cannot replace Fe2+ | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
380000 | - |
ultracentrifugal analysis | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to O2, easily inactivated by aeration | Pseudomonas fluorescens |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.25 | - |
- |
Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, pH 6.0-7.2, in absence of Fe2+, several weeks, stable | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | specific for homogentisate | Pseudomonas fluorescens | 4-maleylacetoacetate | - |
? | |
additional information | not as substrates: salicylic acid, catechol, 2,3-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,6-dihydroxybenzoic acid, o-hydroxyphenylacetic acid, p-hydroxyphenylacetic acid, 3,4-dihydroxyphenylacetic acid | Pseudomonas fluorescens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.2 | -20°C, stable for several weeks | Pseudomonas fluorescens |