Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain Tuner (DE3) | Nitrobacter winogradskyi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting-drop vapor diffusion technique and a nanodropdispensing robot, from 1.0 M Na2HPO4-NaH2PO4, pH 8.2, at 22°C, 1 week, X-ray diffraction structure determination and analysis at 2.10 A resolution | Nitrobacter winogradskyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Nitrobacter winogradskyi | |
0.09 | - |
Chlorite | pH 7.0, 30°C, recombinant enzyme | Nitrobacter winogradskyi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Nitrobacter winogradskyi | 5737 | - |
additional information | the enzyme does not contain a signal peptide for protein translocation into the periplasm and that the cleaving of a signal peptide at the N terminus would severely disturb the structure of the N-terminal domain | Nitrobacter winogradskyi | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme enzyme | Nitrobacter winogradskyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | Nitrobacter winogradskyi | - |
chloride + O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrobacter winogradskyi | Q3SPU6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by nickel affinity chromatography and gel filtration | Nitrobacter winogradskyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorite | - |
Nitrobacter winogradskyi | chloride + O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | subunit interface and active site structures, structure modeling, overview | Nitrobacter winogradskyi |
More | the Cld-like protein from the chemolithoautotrophic nitrite oxidizer Nitrobacter winogradskyi is significantly smaller than all previously known chlorite dismutases | Nitrobacter winogradskyi |
Synonyms | Comment | Organism |
---|---|---|
chlorite dismutase | - |
Nitrobacter winogradskyi |
CLD | - |
Nitrobacter winogradskyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nitrobacter winogradskyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
Chlorite | pH 7.0, 30°C, recombinant enzyme | Nitrobacter winogradskyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Nitrobacter winogradskyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Nitrobacter winogradskyi |
General Information | Comment | Organism |
---|---|---|
additional information | despite a truncated N-terminal domain in each subunit, the dimeric enzyme is a highly efficient chlorite dismutase. Subunit interface and active site structures, structure modeling, overview | Nitrobacter winogradskyi |
physiological function | the enzyme detoxifies the chlorite that results from chlorate respiration in bacteria and would otherwise accumulate in the cell and kill the organism. Nitrobacter has a limited chlorite resistance, which enables it to persist and even oxidize nitrite in the presence of low concentrations of chlorate | Nitrobacter winogradskyi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2111 | - |
Chlorite | pH 7.0, 30°C, recombinant enzyme | Nitrobacter winogradskyi |