BRENDA - Enzyme Database show
show all sequences of 1.13.11.48

Crystallization and preliminary X-ray analysis of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus Rue61a: a cofactor-devoid dioxygenase of the alpha/beta-hydrolase-fold superfamily

Steiner, R.A.; Frerichs-Deeken, U.; Fetzner, S.; Acta Crystallogr. Sect. F 63, 382-385 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression of wild-type and mutant N-terminally His6-tagged HODs in Escherichia coli strain M15
Paenarthrobacter nitroguajacolicus
Crystallization (Commentary)
Crystallization
Organism
N-terminally His6-tagged HOD is crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and CuCl2 as an additive. The structure is solved by the single anomalous dispersion technique using data collected to 3.5 A resolution at the Cu absorption peak wavelength. The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 153.788, c = 120.872 A; purified recombinant wild-type and mutants C69S and C69S/H251A N-terminally His6-tagged HOD, hanging drop vapour diffusion method, 50 mg/ml protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, is mixed with 1.65 M sodium/potassium tartrate, 0.1 M HEPES, pH 7.0, and 30 mM CuCl2, method optimization, X-ray diffraction structure determination and analysis at 3.5 A resolution, single anomalous dispersion technique
Paenarthrobacter nitroguajacolicus
Engineering
Amino acid exchange
Commentary
Organism
C69S
site-directed mutagenesis, the mutant has catalytic properties that are identical to those of wild-type HOD
Paenarthrobacter nitroguajacolicus
C69S/H251A
site-directed mutagenesis, the mutant is catalytically inactive owing to the Ala substitution of the essential residue His251
Paenarthrobacter nitroguajacolicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus
-
N-Acetylanthranilate + CO
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus Rü61a
-
N-Acetylanthranilate + CO
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus Rue61a
-
N-Acetylanthranilate + CO
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paenarthrobacter nitroguajacolicus
-
-
-
Paenarthrobacter nitroguajacolicus Rue61a
-
-
-
Paenarthrobacter nitroguajacolicus Rü61a
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant wild-type and mutant N-terminally His6-tagged HODs from Escherichia coli strain M15 by nickel affinity and anion exchange chromatography
Paenarthrobacter nitroguajacolicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus
N-Acetylanthranilate + CO
-
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus Rü61a
N-Acetylanthranilate + CO
-
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus Rue61a
N-Acetylanthranilate + CO
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
three-dimensional structure analysis, overview
Paenarthrobacter nitroguajacolicus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of wild-type and mutant N-terminally His6-tagged HODs in Escherichia coli strain M15
Paenarthrobacter nitroguajacolicus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
N-terminally His6-tagged HOD is crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and CuCl2 as an additive. The structure is solved by the single anomalous dispersion technique using data collected to 3.5 A resolution at the Cu absorption peak wavelength. The crystals belong to the primitive tetragonal space group P43212, with unit-cell parameters a = b = 153.788, c = 120.872 A; purified recombinant wild-type and mutants C69S and C69S/H251A N-terminally His6-tagged HOD, hanging drop vapour diffusion method, 50 mg/ml protein in 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, is mixed with 1.65 M sodium/potassium tartrate, 0.1 M HEPES, pH 7.0, and 30 mM CuCl2, method optimization, X-ray diffraction structure determination and analysis at 3.5 A resolution, single anomalous dispersion technique
Paenarthrobacter nitroguajacolicus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C69S
site-directed mutagenesis, the mutant has catalytic properties that are identical to those of wild-type HOD
Paenarthrobacter nitroguajacolicus
C69S/H251A
site-directed mutagenesis, the mutant is catalytically inactive owing to the Ala substitution of the essential residue His251
Paenarthrobacter nitroguajacolicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus
-
N-Acetylanthranilate + CO
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus Rü61a
-
N-Acetylanthranilate + CO
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
Paenarthrobacter nitroguajacolicus Rue61a
-
N-Acetylanthranilate + CO
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant wild-type and mutant N-terminally His6-tagged HODs from Escherichia coli strain M15 by nickel affinity and anion exchange chromatography
Paenarthrobacter nitroguajacolicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus
N-Acetylanthranilate + CO
-
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus Rü61a
N-Acetylanthranilate + CO
-
-
-
?
1H-3-Hydroxy-4-oxoquinaldine + O2
-
684175
Paenarthrobacter nitroguajacolicus Rue61a
N-Acetylanthranilate + CO
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
three-dimensional structure analysis, overview
Paenarthrobacter nitroguajacolicus
Other publictions for EC 1.13.11.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742251
Pietra
Binding pockets and permeatio ...
Paenarthrobacter nitroguajacolicus
Chem. Biodivers.
11
861-871
2014
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696277
Boehm
Thermodynamic analysis of dena ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus R-61a, Paenarthrobacter nitroguajacolicus Rü61a
Biochemistry
47
7116-7126
2008
-
-
1
-
1
-
2
1
-
-
1
4
-
5
-
-
1
-
-
-
1
-
4
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
4
-
1
-
-
1
4
-
-
-
1
-
-
1
-
4
1
1
-
2
-
-
-
-
-
-
1
1
-
-
-
684175
Steiner
Crystallization and preliminar ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus Rü61a, Paenarthrobacter nitroguajacolicus Rue61a
Acta Crystallogr. Sect. F
63
382-385
2007
-
-
1
1
2
-
-
-
-
-
-
3
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714163
Beermann
Stability, unfolding, and stru ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus Rü61a
Biochemistry
46
4241-4249
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
673207
Frerichs-Deeken
Dioxygenases without requireme ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus R-61a, Paenarthrobacter nitroguajacolicus Rü61a
Curr. Microbiol.
51
344-352
2005
-
-
1
-
17
-
-
18
-
-
-
3
-
4
-
-
1
1
-
-
-
-
12
-
1
-
-
20
1
2
1
-
-
-
-
-
-
1
-
-
17
-
-
-
-
18
-
-
-
3
-
-
-
1
-
-
-
-
12
-
1
-
-
20
1
2
1
-
-
-
-
-
-
-
658042
Frerichs-Deeken
Dioxygenases without requireme ...
Paenarthrobacter ilicis, Paenarthrobacter ilicis Ru61a
Biochemistry
43
14485-14499
2004
-
-
1
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
285259
Fischer
Bacterial 2,4-dioxygenases: ne ...
Arthrobacter sp.
J. Bacteriol.
181
5725-5733
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207895
Bauer
2,4-Dioxygenases catalyzing N- ...
Arthrobacter sp., Arthrobacter sp. Ru61a
Eur. J. Biochem.
240
576-583
1996
-
-
-
-
-
-
8
1
-
-
1
-
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
1
-
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207896
Bauer
-
A novel type of oxygenolytic r ...
Arthrobacter sp., Arthrobacter sp. Ru61a
FEMS Microbiol. Lett.
117
299-304
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-