BRENDA - Enzyme Database show
show all sequences of 1.13.11.48

2,4-Dioxygenases catalyzing N-heterocyclic-ring cleavage and formation of carbon monoxide. Purification and some properties of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter sp. Rii61a and comparison with 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from Pseudomonas putida 33/1

Bauer, I.; Max, N.; Fetzner, S.; Lingens, F.; Eur. J. Biochem. 240, 576-583 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, weak inhibition
Arthrobacter sp.
Co2+
1 mM, weak inhibition
Arthrobacter sp.
Cu2+
1 mM, strong inhibition
Arthrobacter sp.
Fe2+
1 mM, strong inhibition
Arthrobacter sp.
Mg2+
1 mM, weak inhibition
Arthrobacter sp.
Mn2+
1 mM, weak inhibition
Arthrobacter sp.
Ni2+
1 mM, strong inhibition
Arthrobacter sp.
Zn2+
1 mM, strong inhibition
Arthrobacter sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
1H-3-Hydroxy-4-oxoquinaldine
-
Arthrobacter sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Arthrobacter sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arthrobacter sp.
-
Ru61a
-
Arthrobacter sp. Ru61a
-
Ru61a
-
Purification (Commentary)
Commentary
Organism
-
Arthrobacter sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
70.7
-
-
Arthrobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1H-3-hydroxy-4-oxoquinaldine + O2
1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp.
N-acetylanthranilic acid + CO
-
207895
Arthrobacter sp.
ir
1H-3-hydroxy-4-oxoquinaldine + O2
1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp. Ru61a
N-acetylanthranilic acid + CO
-
207895
Arthrobacter sp. Ru61a
ir
1H-3-hydroxy-4-oxoquinoline + O2
also 1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp.
N-formylanthranilic acid + CO
-
207895
Arthrobacter sp.
ir
1H-3-hydroxy-4-oxoquinoline + O2
also 1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp. Ru61a
N-formylanthranilic acid + CO
-
207895
Arthrobacter sp. Ru61a
ir
Subunits
Subunits
Commentary
Organism
monomer
SDS-PAGE
Arthrobacter sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactors
Arthrobacter sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactors
Arthrobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
1 mM, weak inhibition
Arthrobacter sp.
Co2+
1 mM, weak inhibition
Arthrobacter sp.
Cu2+
1 mM, strong inhibition
Arthrobacter sp.
Fe2+
1 mM, strong inhibition
Arthrobacter sp.
Mg2+
1 mM, weak inhibition
Arthrobacter sp.
Mn2+
1 mM, weak inhibition
Arthrobacter sp.
Ni2+
1 mM, strong inhibition
Arthrobacter sp.
Zn2+
1 mM, strong inhibition
Arthrobacter sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
1H-3-Hydroxy-4-oxoquinaldine
-
Arthrobacter sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
gel filtration
Arthrobacter sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arthrobacter sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
70.7
-
-
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1H-3-hydroxy-4-oxoquinaldine + O2
1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp.
N-acetylanthranilic acid + CO
-
207895
Arthrobacter sp.
ir
1H-3-hydroxy-4-oxoquinaldine + O2
1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp. Ru61a
N-acetylanthranilic acid + CO
-
207895
Arthrobacter sp. Ru61a
ir
1H-3-hydroxy-4-oxoquinoline + O2
also 1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp.
N-formylanthranilic acid + CO
-
207895
Arthrobacter sp.
ir
1H-3-hydroxy-4-oxoquinoline + O2
also 1H-3-hydroxy-4-oxoquinaldine oxygenase
207895
Arthrobacter sp. Ru61a
N-formylanthranilic acid + CO
-
207895
Arthrobacter sp. Ru61a
ir
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
SDS-PAGE
Arthrobacter sp.
Other publictions for EC 1.13.11.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696277
Boehm
Thermodynamic analysis of dena ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus R-61a, Paenarthrobacter nitroguajacolicus Rü61a
Biochemistry
47
7116-7126
2008
-
-
1
-
1
-
2
1
-
-
1
4
-
5
-
-
1
-
-
-
1
-
4
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
4
-
1
-
-
1
4
-
-
-
1
-
-
1
-
4
1
1
-
2
-
-
-
-
-
-
1
1
-
-
-
684175
Steiner
Crystallization and preliminar ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus Rü61a, Paenarthrobacter nitroguajacolicus Rue61a
Acta Crystallogr. Sect. F
63
382-385
2007
-
-
1
1
2
-
-
-
-
-
-
3
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714163
Beermann
Stability, unfolding, and stru ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus Rü61a
Biochemistry
46
4241-4249
2007
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
673207
Frerichs-Deeken
Dioxygenases without requireme ...
Paenarthrobacter nitroguajacolicus, Paenarthrobacter nitroguajacolicus R-61a, Paenarthrobacter nitroguajacolicus Rü61a
Curr. Microbiol.
51
344-352
2005
-
-
1
-
17
-
-
18
-
-
-
3
-
4
-
-
1
1
-
-
-
-
12
-
1
-
-
20
1
2
1
-
-
-
-
-
-
1
-
-
17
-
-
-
-
18
-
-
-
3
-
-
-
1
-
-
-
-
12
-
1
-
-
20
1
2
1
-
-
-
-
-
-
-
658042
Frerichs-Deeken
Dioxygenases without requireme ...
Paenarthrobacter ilicis, Paenarthrobacter ilicis Ru61a
Biochemistry
43
14485-14499
2004
-
-
1
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
285259
Fischer
Bacterial 2,4-dioxygenases: ne ...
Arthrobacter sp.
J. Bacteriol.
181
5725-5733
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207895
Bauer
2,4-Dioxygenases catalyzing N- ...
Arthrobacter sp., Arthrobacter sp. Ru61a
Eur. J. Biochem.
240
576-583
1996
-
-
-
-
-
-
8
1
-
-
1
-
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
8
-
1
-
-
1
-
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207896
Bauer
-
A novel type of oxygenolytic r ...
Arthrobacter sp., Arthrobacter sp. Ru61a
FEMS Microbiol. Lett.
117
299-304
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-