Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Amycolatopsis orientalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | putative FeIV=O species hydroxylates benzylic position | Amycolatopsis orientalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40368 | - |
alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry | Amycolatopsis orientalis |
80000 | - |
gel filtration, electrospray mass spectrometry | Amycolatopsis orientalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Amycolatopsis orientalis | - |
4-hydroxymandelate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged protein, Ni-NTA resin | Amycolatopsis orientalis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylpyruvate + O2 = (S)-4-hydroxymandelate + CO2 | one oxygen from molecular oxygen is incorporated into the carboxyl group and one into the benzylic hydroxyl group | Amycolatopsis orientalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Amycolatopsis orientalis | 4-hydroxymandelate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry | Amycolatopsis orientalis |