BRENDA - Enzyme Database show
show all sequences of 1.13.11.45

Expression of manganese lipoxygenase in Pichia pastoris and site-directed mutagenesis of putative metal ligands

Cristea, M.; Engstrom, K.; Su, C.; Hornsten, L.; Oliw, E.H.; Arch. Biochem. Biophys. 434, 201-211 (2005)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
H274Q
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H278E
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H462E
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H463Q
no significant change in activity
Gaeumannomyces graminis
additional information
V602 deletion mutant, no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
N466L
no significant change in activity
Gaeumannomyces graminis
N466Q
no significant change in activity
Gaeumannomyces graminis
Q467N
no significant change in activity
Gaeumannomyces graminis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
alpha-linolenic acid
pH 9.0, 21C
Gaeumannomyces graminis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
1 mol per mol of protein
Gaeumannomyces graminis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gaeumannomyces graminis
-
expression in Pichia pastoris
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
N-and O-glycosilation
Gaeumannomyces graminis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Gaeumannomyces graminis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
18
-
pH 9.0, 21C
Gaeumannomyces graminis
Storage Stability
Storage Stability
Organism
4C, stable for months
Gaeumannomyces graminis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + O2
-
654377
Gaeumannomyces graminis
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 80-100000, SDS-PAGE
Gaeumannomyces graminis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Gaeumannomyces graminis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
-
93% of maximum activity
Gaeumannomyces graminis
8
-
98% of maximum activity
Gaeumannomyces graminis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H274Q
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H278E
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H462E
no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
H463Q
no significant change in activity
Gaeumannomyces graminis
additional information
V602 deletion mutant, no enzymic activity, loss of more than 95% of manganese content
Gaeumannomyces graminis
N466L
no significant change in activity
Gaeumannomyces graminis
N466Q
no significant change in activity
Gaeumannomyces graminis
Q467N
no significant change in activity
Gaeumannomyces graminis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0071
-
alpha-linolenic acid
pH 9.0, 21C
Gaeumannomyces graminis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
1 mol per mol of protein
Gaeumannomyces graminis
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
N-and O-glycosilation
Gaeumannomyces graminis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture supernatant
-
Gaeumannomyces graminis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
18
-
pH 9.0, 21C
Gaeumannomyces graminis
Storage Stability (protein specific)
Storage Stability
Organism
4C, stable for months
Gaeumannomyces graminis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-linolenic acid + O2
-
654377
Gaeumannomyces graminis
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80-100000, SDS-PAGE
Gaeumannomyces graminis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Gaeumannomyces graminis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
-
93% of maximum activity
Gaeumannomyces graminis
8
-
98% of maximum activity
Gaeumannomyces graminis
Other publictions for EC 1.13.11.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743826
Newie
Lipoxygenase 2 from Cyanothec ...
Cyanothece sp. PCC 8801
Sci. Rep.
7
2069
2017
-
-
1
1
6
-
-
-
-
1
-
1
-
2
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
6
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
741777
Wennman
Expression and characterizati ...
Magnaporthe oryzae, Magnaporthe oryzae 70-15
Arch. Biochem. Biophys.
583
87-95
2015
-
-
1
-
-
-
-
1
-
3
-
-
-
6
-
1
1
-
-
-
1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
-
-
-
1
1
-
-
1
-
10
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
743055
Wennman
Manganese lipoxygenase of F. ...
Colletotrichum gloeosporioides, Fusarium oxysporum
J. Lipid Res.
56
1606-1615
2015
-
-
1
-
3
-
2
5
1
6
-
-
-
6
-
-
1
-
-
-
-
-
10
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
2
-
5
1
6
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
1
1
-
-
-
-
3
3
-
-
-
725658
Wennman
Secretion of two novel enzymes ...
Gaeumannomyces graminis
J. Lipid Res.
54
762-775
2013
-
-
1
-
4
1
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
4
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
724444
Hoffmann
Novel insights into cyclooxyge ...
Glycine max
Biochim. Biophys. Acta
1821
1508-1517
2012
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
725486
Wennman
Catalytic convergence of manga ...
Gaeumannomyces graminis
J. Biol. Chem.
287
31757-31765
2012
-
-
1
-
10
-
-
5
-
1
-
3
-
2
-
-
1
-
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
10
-
-
-
-
5
-
1
-
3
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
688235
Oliw
Factors influencing the rearra ...
Gaeumannomyces graminis
J. Lipid Res.
49
420-428
2008
-
-
1
-
3
-
-
1
1
2
-
-
-
1
-
-
-
1
-
-
-
-
9
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
688224
Cristea
On the singular, dual, and mul ...
Gaeumannomyces graminis
J. Lipid Res.
48
890-903
2007
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
-
1
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674584
Cristea
A G316A mutation of manganese ...
Gaeumannomyces graminis
J. Biol. Chem.
281
17612-17623
2006
-
-
1
-
3
-
2
1
-
1
-
-
-
2
-
-
1
-
-
-
-
-
2
-
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-
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-
-
-
-
-
-
-
1
-
-
3
-
-
2
-
1
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654377
Cristea
Expression of manganese lipoxy ...
Gaeumannomyces graminis
Arch. Biochem. Biophys.
434
201-211
2005
-
-
-
-
8
-
-
1
-
1
-
-
-
5
-
1
-
-
-
1
1
1
1
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
1
-
1
-
-
-
-
1
-
-
1
1
1
1
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
656687
Oliw
Biosynthesis and isomerization ...
Gaeumannomyces graminis
Lipids
39
319-323
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
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-
3
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-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654157
Su
Manganese lipoxygenase has a m ...
Gaeumannomyces graminis
Adv. Exp. Med. Biol.
507
171-176
2002
-
-
-
-
-
-
-
-
-
1
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-
1
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-
2
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-
1
-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285253
Su
Manganese lipoxygenase. Purifi ...
Gaeumannomyces graminis
J. Biol. Chem.
273
13072-13079
1998
-
-
-
-
-
-
4
3
-
1
3
-
-
1
-
1
1
-
-
-
-
1
3
-
1
1
1
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
3
-
1
3
-
-
-
1
1
-
-
-
1
3
-
1
1
1
3
1
1
-
-
-
-
-
-
-
-
285254
Hamberg
Manganese lipoxygenase. Discov ...
Gaeumannomyces graminis
J. Biol. Chem.
273
13080-13088
1998
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
1
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-
1
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-
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-
-
-
-
285252
Koch
Calcium requirement for lipoxy ...
Glycine max, Phaseolus vulgaris
J. Am. Oil Chem. Soc.
48
532-538
1971
-
-
-
-
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-
1
-
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4
-
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2
-
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-
-
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2
-
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-
2
2
-
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1
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4
-
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2
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2
2
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