BRENDA - Enzyme Database show
show all sequences of 1.13.11.43

Purification and some properties of lignostilbene-alpha,beta-dioxygenase responsible for the Calpha-Cbeta cleavage of a diarylpropane type lignin model compound from Pseudomonas sp. TMY1009

Kamoda, S.; Habu, N.; Samejima, M.; Yoshimoto, T.; Agric. Biol. Chem. 53, 2757-2761 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene
isozyme I
Sphingomonas paucimobilis
0.11
-
O2
isozyme I
Sphingomonas paucimobilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Sphingomonas paucimobilis
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe
1 g-atom per mol enzyme
Sphingomonas paucimobilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha2, 2 * 52000, SDS-PAGE, isozyme I
Sphingomonas paucimobilis
94000
-
gel filtration, isozyme I, II, III
Sphingomonas paucimobilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
stilbene + O2
Sphingomonas paucimobilis
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
?
-
-
?
stilbene + O2
Sphingomonas paucimobilis TMY 1009
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingomonas paucimobilis
-
formerly Pseudomonas sp.; four isoenzymes
-
Sphingomonas paucimobilis TMY 1009
-
four isoenzymes
-
Purification (Commentary)
Commentary
Organism
major isozyme I, 4 isozymes by ion-exchange chromatography
Sphingomonas paucimobilis
Storage Stability
Storage Stability
Organism
4C, one week, 70% activity, isozyme I
Sphingomonas paucimobilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
stilbene-type intermediate of diarylpropane-type lignin model compound
440285
Sphingomonas paucimobilis
vanillin
-
440285
Sphingomonas paucimobilis
?
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
stilbene-type intermediate of diarylpropane-type lignin model compound
440285
Sphingomonas paucimobilis TMY 1009
vanillin
-
440285
Sphingomonas paucimobilis TMY 1009
?
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440285
Sphingomonas paucimobilis
?
-
-
-
-
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
-
stilbene + O2
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
440285
Sphingomonas paucimobilis
?
-
-
-
?
stilbene + O2
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
trans-4-hydroxystilbene + O2
oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation
440285
Sphingomonas paucimobilis
?
-
-
-
?
trans-4-hydroxystilbene + O2
oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
alpha2, 2 * 52000, SDS-PAGE, isozyme I
Sphingomonas paucimobilis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
after 3 h, 100% activity retained, isozyme 1
Sphingomonas paucimobilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
isozyme I
Sphingomonas paucimobilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene
isozyme I
Sphingomonas paucimobilis
0.11
-
O2
isozyme I
Sphingomonas paucimobilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Sphingomonas paucimobilis
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe
1 g-atom per mol enzyme
Sphingomonas paucimobilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha2, 2 * 52000, SDS-PAGE, isozyme I
Sphingomonas paucimobilis
94000
-
gel filtration, isozyme I, II, III
Sphingomonas paucimobilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
stilbene + O2
Sphingomonas paucimobilis
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
?
-
-
?
stilbene + O2
Sphingomonas paucimobilis TMY 1009
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
major isozyme I, 4 isozymes by ion-exchange chromatography
Sphingomonas paucimobilis
Storage Stability (protein specific)
Storage Stability
Organism
4C, one week, 70% activity, isozyme I
Sphingomonas paucimobilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
stilbene-type intermediate of diarylpropane-type lignin model compound
440285
Sphingomonas paucimobilis
vanillin
-
440285
Sphingomonas paucimobilis
?
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
stilbene-type intermediate of diarylpropane-type lignin model compound
440285
Sphingomonas paucimobilis TMY 1009
vanillin
-
440285
Sphingomonas paucimobilis TMY 1009
?
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440285
Sphingomonas paucimobilis
?
-
-
-
-
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
-
stilbene + O2
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
440285
Sphingomonas paucimobilis
?
-
-
-
?
stilbene + O2
reaction in bacterial lignin degradation, responsible for degradation of a diarylpropane-type structure in lignin
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
trans-4-hydroxystilbene + O2
oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation
440285
Sphingomonas paucimobilis
?
-
-
-
?
trans-4-hydroxystilbene + O2
oxidation at 29% the rate of 1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene oxidation
440285
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
alpha2, 2 * 52000, SDS-PAGE, isozyme I
Sphingomonas paucimobilis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
-
after 3 h, 100% activity retained, isozyme 1
Sphingomonas paucimobilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
isozyme I
Sphingomonas paucimobilis
Other publictions for EC 1.13.11.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672665
Kamoda
Production of heterogeneous di ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY1009
Biosci. Biotechnol. Biochem.
69
635-637
2005
-
-
1
-
-
-
-
-
-
-
-
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-
4
-
-
1
-
-
-
2
-
4
1
-
-
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1
-
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1
-
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2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655038
Kamoda
A common structure of substrat ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY1009
Biosci. Biotechnol. Biochem.
67
1394-1396
2003
-
-
-
-
-
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-
1
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3
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6
-
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1
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6
-
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656384
Han
N-benzylideneaniline and N-ben ...
Sphingomonas paucimobilis
J. Enzyme Inhib. Med. Chem.
18
279-283
2003
-
-
-
-
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-
3
1
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1
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1
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1
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3
1
1
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1
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-
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-
440280
Makoto
-
Reactivity and stability of li ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
J. Microbiol. Biotechnol.
11
884-886
2001
1
-
-
-
-
-
-
-
-
-
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1
7
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-
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-
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1
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1
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1
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1
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1
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1
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1
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1
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-
440281
Kamoda
Purification and some properti ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
61
1575-1576
1997
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-
-
-
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2
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2
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7
-
-
1
-
-
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4
1
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2
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2
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1
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4
1
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-
-
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-
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-
-
-
-
-
-
-
-
440282
Kamoda
Cloning of a lignostilbene-alp ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
59
1866-1868
1995
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-
1
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-
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1
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7
-
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1
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2
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1
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1
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2
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440283
Kamoda
Structural and enzymic compari ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
57
931-934
1993
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-
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2
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7
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1
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8
2
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2
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1
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8
2
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440284
Kamoda
Cloning of a lignostilbene-alp ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Agric. Biol. Chem.
55
1411-1412
1991
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1
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7
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2
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1
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2
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440285
Kamoda
-
Purification and some properti ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Agric. Biol. Chem.
53
2757-2761
1989
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2
1
1
2
2
-
7
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1
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1
8
1
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1
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1
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2
1
1
2
2
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1
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1
8
1
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1
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1
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