BRENDA - Enzyme Database show
show all sequences of 1.13.11.43

Structural and enzymic comparison of lignostilbene-alpha,beta-dioxygenase isozymes, I, II, and III, from Pseudomonas paucimobilis TMY1009

Kamoda, S.; Saburi, Y.; Biosci. Biotechnol. Biochem. 57, 931-934 (1993)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha, beta, 1 * 52000 + 1 * 52000, SDS-PAGE, isozyme II; beta2, 2 * 52000, SDS-PAGE, isozyme III
Sphingomonas paucimobilis
94000
-
gel filtration, isozyme I, II, III
Sphingomonas paucimobilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingomonas paucimobilis
-
four isoenzymes
-
Sphingomonas paucimobilis TMY 1009
-
four isoenzymes
-
Purification (Commentary)
Commentary
Organism
isozymes I, II and III
Sphingomonas paucimobilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis
vanillin
-
-
-
?
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis TMY 1009
vanillin
-
-
-
?
4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
best activity with isozyme III
440283
Sphingomonas paucimobilis
?
-
-
-
?
4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
best activity with isozyme III
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440283
Sphingomonas paucimobilis
?
-
-
-
-
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
-
trans-4-hydroxystilbene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis
?
-
-
-
?
trans-4-hydroxystilbene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
alpha, beta, 1 * 52000 + 1 * 52000, SDS-PAGE, isozyme II
Sphingomonas paucimobilis
homodimer
beta2, 2 * 52000, SDS-PAGE, isozyme III
Sphingomonas paucimobilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
52000
-
alpha, beta, 1 * 52000 + 1 * 52000, SDS-PAGE, isozyme II; beta2, 2 * 52000, SDS-PAGE, isozyme III
Sphingomonas paucimobilis
94000
-
gel filtration, isozyme I, II, III
Sphingomonas paucimobilis
Purification (Commentary) (protein specific)
Commentary
Organism
isozymes I, II and III
Sphingomonas paucimobilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis
vanillin
-
-
-
?
1,2-bis(4'-hydroxy-3'-methoxyphenyl)ethylene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis TMY 1009
vanillin
-
-
-
?
4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
best activity with isozyme III
440283
Sphingomonas paucimobilis
?
-
-
-
?
4,2'-dihydroxy-3,3'-dimethoxy-5'-(2''-carboxyvinyl)stilbene + O2
best activity with isozyme III
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440283
Sphingomonas paucimobilis
?
-
-
-
-
additional information
catalyzes oxidative cleavage of the interphenyl double bond in the synthetic substrate and lignin-derived stilbenes, poor substrates are 5-(2'-(4''-hydroxy-3''-methoxy-phenyl)-vinyl)ferulic acid, 4-hydroxy-3-methoxystyrene, isoeugenol
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
-
trans-4-hydroxystilbene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis
?
-
-
-
?
trans-4-hydroxystilbene + O2
best activity with isozyme I
440283
Sphingomonas paucimobilis TMY 1009
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
alpha, beta, 1 * 52000 + 1 * 52000, SDS-PAGE, isozyme II
Sphingomonas paucimobilis
homodimer
beta2, 2 * 52000, SDS-PAGE, isozyme III
Sphingomonas paucimobilis
Other publictions for EC 1.13.11.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672665
Kamoda
Production of heterogeneous di ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY1009
Biosci. Biotechnol. Biochem.
69
635-637
2005
-
-
1
-
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4
-
-
1
-
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2
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4
1
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1
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1
-
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2
-
4
1
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-
-
655038
Kamoda
A common structure of substrat ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY1009
Biosci. Biotechnol. Biochem.
67
1394-1396
2003
-
-
-
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-
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1
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3
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6
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1
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6
-
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-
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-
-
-
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-
-
-
-
656384
Han
N-benzylideneaniline and N-ben ...
Sphingomonas paucimobilis
J. Enzyme Inhib. Med. Chem.
18
279-283
2003
-
-
-
-
-
-
3
1
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1
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1
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1
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3
1
1
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1
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-
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-
440280
Makoto
-
Reactivity and stability of li ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
J. Microbiol. Biotechnol.
11
884-886
2001
1
-
-
-
-
-
-
-
-
-
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1
7
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
1
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1
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1
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1
-
1
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-
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-
-
-
440281
Kamoda
Purification and some properti ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
61
1575-1576
1997
-
-
-
-
-
-
-
2
-
-
2
-
-
7
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
-
-
-
-
-
2
-
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2
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-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
440282
Kamoda
Cloning of a lignostilbene-alp ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
59
1866-1868
1995
-
-
1
-
-
-
-
-
-
-
1
-
-
7
-
-
1
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
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1
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1
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1
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2
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-
-
-
-
-
-
-
-
-
-
-
-
440283
Kamoda
Structural and enzymic compari ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Biosci. Biotechnol. Biochem.
57
931-934
1993
-
-
-
-
-
-
-
-
-
-
2
-
-
7
-
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1
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8
2
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2
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1
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8
2
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-
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-
-
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-
440284
Kamoda
Cloning of a lignostilbene-alp ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Agric. Biol. Chem.
55
1411-1412
1991
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-
1
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-
-
-
-
-
-
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-
7
-
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-
-
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2
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1
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2
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440285
Kamoda
-
Purification and some properti ...
Sphingomonas paucimobilis, Sphingomonas paucimobilis TMY 1009
Agric. Biol. Chem.
53
2757-2761
1989
-
-
-
-
-
-
-
2
1
1
2
2
-
7
-
-
1
-
-
-
-
1
8
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
2
-
-
-
1
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-
1
8
1
-
-
1
-
1
-
-
-
-
-
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