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Literature summary for 1.13.11.41 extracted from

  • Roberts, K.M.; Connor, G.C.; Cave, C.H.; Rowe, G.T.; Page, C.A.
    The metal- and substrate-dependences of 2,4'-dihydroxyacetophenone dioxygenase (2020), Arch. Biochem. Biophys., 691, 108441 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.41

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzyme V1M is expressed in Escherichia coli BL21(DE3) cells Burkholderia sp. AZ11

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxybenzoate product inhibition Burkholderia sp. AZ11
Cu2+
-
 Burkholderia sp. AZ11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00063
-
 2,4'-dihydroxyacetophenone mutant enzyme V1M, at pH 6.0 and 20°C Burkholderia sp. AZ11

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ anaerobic reconstitution of the purified enzyme with five equivalents of Fe2+ results in an increase in activity of 7% which increases to 13% upon overnight incubation in air Burkholderia sp. AZ11
Fe3+ the enzyme contains a Fe3+ center Burkholderia sp. AZ11
additional information incubation of apoenzyme with Ca2+, Mg2+, Mn2+, Ni2+, or Zn2+ results in no effect on the background activity after either 2 h or overnight Burkholderia sp. AZ11

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4'-dihydroxyacetophenone + O2 Burkholderia sp. AZ11
-
 4-hydroxybenzoate + formate
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia sp. AZ11
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Burkholderia sp. AZ11

Storage Stability

Storage Stability Organism
4°C, purified enzyme in 50 mM potassium phosphate buffer pH 7.0, several months, the enzyme remains stable Burkholderia sp. AZ11

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4'-dihydroxyacetophenone + O2
-
 Burkholderia sp. AZ11 4-hydroxybenzoate + formate
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 20000, SDS-PAGE Burkholderia sp. AZ11

Synonyms

Synonyms Comment Organism
DAD
-
 Burkholderia sp. AZ11

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.32
-
 O2 mutant enzyme V1M, at pH 6.0 and 20°C Burkholderia sp. AZ11
2.42
-
 2,4'-dihydroxyacetophenone mutant enzyme V1M, at pH 6.0 and 20°C Burkholderia sp. AZ11

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1
-
 4-hydroxybenzoate mutant enzyme V1M, at pH 6.0 and 20°C Burkholderia sp. AZ11

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3800
-
 2,4'-dihydroxyacetophenone mutant enzyme V1M, at pH 6.0 and 20°C Burkholderia sp. AZ11