Literature summary for 1.13.11.41 extracted from

Enya, M.; Aoyagi, K.; Hishikawa, Y.; Yoshimura, A.; Mitsukura, K.; Maruyama, K.
Molecular and catalytic properties of 2,4'-dihydroxyacetophenone dioxygenase from Burkholderia sp. AZ11 (2012), Biosci. Biotechnol. Biochem., 76, 567-574.

Search for more literature for 1.13.11.41

Activating Compound

Activating Compound	Comment	Organism	Structure
2-mercaptoethanol	slight activation	Burkholderia sp.

Cloned(Commentary)

Cloned (Comment)	Organism
gene dad, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain JM109	Burkholderia sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Burkholderia sp.
2,2'-dipyridine	-	Burkholderia sp.
4-chloromercuribenzoate	-	Burkholderia sp.
8-hydroxyquinoline	-	Burkholderia sp.
Co2+	-	Burkholderia sp.
Cu2+	strong inhibition	Burkholderia sp.
diethyldicarbonate	-	Burkholderia sp.
Hg2+	strong inhibition	Burkholderia sp.
Ni2+	-	Burkholderia sp.
Tiron	-	Burkholderia sp.
Zn2+	-	Burkholderia sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0016	-	2,4'-dihydroxyacetophenone	pH 7.0, 25°C, recombinant enzyme	Burkholderia sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains 1.69 mol of non-heme iron per mol of enzyme, His77, His79, His115, and Glu96 in the cupin fold are putative metal ligands, Fe2+ weakly activates the enzyme	Burkholderia sp.
additional information	poor or no effects by Fe3+, Ca2+ and Mg2+	Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
23000	-	4 * 23000, recombinant enzyme, SDS-PAGE	Burkholderia sp.
90000	-	recombinant enzyme, gel filtration	Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,4'-dihydroxyacetophenone + O2	Burkholderia sp.	-	4-hydroxybenzoate + formate	-	?

Organism

Organism	UniProt	Comment	Textmining
Burkholderia sp.	F7J5X4	isolated from a soil ssampple in Gifu, Japan, gene dad	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme 39fold from Escherichia coli strain JM109 by heat treatment, ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, native enzyme 17fold from strain AZ11 by gel filtration, anion exchange and hydrophobic interaction chromatography	Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.042	-	crude enzyme extract of strain AZ11, pH 7.0, 25°C	Burkholderia sp.
0.33	-	crude enzyme extract of recombinant Escherichia coli strain JM109 expressing gene dad, pH 7.0, 25°C	Burkholderia sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,4'-dihydroxyacetophenone + O2	-	Burkholderia sp.	4-hydroxybenzoate + formate	-	?
2,4'-dihydroxyacetophenone + O2	anaerobic conditions	Burkholderia sp.	4-hydroxybenzoate + formate	-	?
additional information	2-hydroxyacetophenone is a poor substrate	Burkholderia sp.	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	4 * 23000, recombinant enzyme, SDS-PAGE	Burkholderia sp.

Synonyms

Synonyms	Comment	Organism
DAD	-	Burkholderia sp.
DHAP dioxygenase	-	Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Burkholderia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	purified enzyme, 20 min, over 80% activity remaining	Burkholderia sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	7	-	Burkholderia sp.

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in degradation of bisphenol A	Burkholderia sp.
physiological function	the enzyme is involved in degradation of bisphenol A	Burkholderia sp.

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Release 2023.1 (January 2023)