Application | Comment | Organism |
---|---|---|
analysis | high potential for use in biosensor technologies for monitoring the metabolism of tyrosine and salicylic acid, as well as a pollution of environment and different materials by polyaromatic compounds | Rhodococcus opacus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21 (DE3) | Rhodococcus opacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Dihydroxybenzoate | - |
Rhodococcus opacus | |
2,4-Dihydroxybenzoate | - |
Rhodococcus opacus | |
2,6-dihydroxybenzoate | - |
Rhodococcus opacus | |
4-Chlorocatechol | - |
Rhodococcus opacus | |
4-methylcatechol | - |
Rhodococcus opacus | |
5-chlorosalicylate | - |
Rhodococcus opacus | |
catechol | - |
Rhodococcus opacus | |
homogentisate | - |
Rhodococcus opacus | |
Hydroxyquinol | - |
Rhodococcus opacus | |
salicylate | - |
Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
2,5-Dihydroxybenzoate | pH 8.5, 25°C | Rhodococcus opacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39600 | - |
calculated from sequence | Rhodococcus opacus |
177000 | - |
gel filtration | Rhodococcus opacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | Rhodococcus opacus | - |
maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | Rhodococcus opacus 1CP | - |
maleylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | W5U174 | - |
- |
Rhodococcus opacus 1CP | W5U174 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Rhodococcus opacus | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | narrow substrate specificity | Rhodococcus opacus | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Rhodococcus opacus 1CP | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | narrow substrate specificity | Rhodococcus opacus 1CP | maleylpyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Rhodococcus opacus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Rhodococcus opacus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8 | in 50 mM Tris/H2SO4 buffer | Rhodococcus opacus |
8 | - |
in 50 mM Clark and Lubs phosphate buffer | Rhodococcus opacus |
8 | 8.3 | in 50 mM Tris/HCl buffer | Rhodococcus opacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00145 | - |
4-Chlorocatechol | pH 8.5, 25°C | Rhodococcus opacus | |
0.0067 | - |
4-methylcatechol | pH 8.5, 25°C | Rhodococcus opacus | |
0.0449 | - |
catechol | pH 8.5, 25°C | Rhodococcus opacus | |
0.057 | - |
5-chlorosalicylate | pH 8.5, 25°C | Rhodococcus opacus | |
0.22 | - |
2,6-dihydroxybenzoate | pH 8.5, 25°C | Rhodococcus opacus | |
0.33 | - |
Hydroxyquinol | pH 8.5, 25°C | Rhodococcus opacus | |
0.444 | - |
homogentisate | pH 8.5, 25°C | Rhodococcus opacus | |
0.81 | - |
salicylate | pH 8.5, 25°C | Rhodococcus opacus | |
1.069 | - |
2,3-Dihydroxybenzoate | pH 8.5, 25°C | Rhodococcus opacus | |
1.13 | - |
2,4-Dihydroxybenzoate | pH 8.5, 25°C | Rhodococcus opacus |