Literature summary for 1.13.11.4 extracted from

Huang, L.; Hu, H.; Tang, H.; Liu, Y.; Xu, P.; Shi, J.; Lin, K.; Luo, Q.; Cui, C.
Identification and characterization of a novel gentisate 1,2-dioxygenase gene from a halophilic Martelella strain (2015), Sci. Rep., 5, 14307 .

Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Martelella sp. AD-3

Protein Variants

Protein Variants	Comment	Organism
H155A	inactive	Martelella sp. AD-3
H157A	inactive	Martelella sp. AD-3
H167A	inactive	Martelella sp. AD-3
H169A	inactive	Martelella sp. AD-3

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	the addition of 0.005 mM Co2+ does inhibit enzyme activity to 53%	Martelella sp. AD-3

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0264	-	2,5-Dihydroxybenzoate	at pH 7.0 and 23°C	Martelella sp. AD-3

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	activates	Martelella sp. AD-3
Fe2+	activates	Martelella sp. AD-3
Mn2+	activates	Martelella sp. AD-3
additional information	Ca2+, Ni2+, Mg2+, and Zn2+ have no impact on enzyme activity	Martelella sp. AD-3

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
120000	-	gel filtration	Martelella sp. AD-3

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,5-dihydroxybenzoate + O2	Martelella sp. AD-3	-	maleylpyruvate	-	?

Organism

Organism	UniProt	Comment	Textmining
Martelella sp. AD-3	A0A127CMI7	-	-

Storage Stability

Storage Stability	Organism
-20°C, purified enzyme, 98 h, complete loss of activity	Martelella sp. AD-3

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,5-dihydroxybenzoate + O2	-	Martelella sp. AD-3	maleylpyruvate	-	?

Subunits

Subunits	Comment	Organism
homotrimer	3 * 38000, SDS-PAGE	Martelella sp. AD-3

Synonyms

Synonyms	Comment	Organism
GDO	-	Martelella sp. AD-3

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Martelella sp. AD-3

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
161.29	-	2,5-Dihydroxybenzoate	at pH 7.0 and 23°C	Martelella sp. AD-3

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Martelella sp. AD-3

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Release 2023.1 (January 2023)