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Literature summary for 1.13.11.4 extracted from

  • Eppinger, E.; Stolz, A.
    Expansion of the substrate range of the gentisate 1,2-dioxygenase from Corynebacterium glutamicum for the conversion of monohydroxylated benzoates (2017), Protein Eng. Des. Sel., 30, 57-65 .
    View publication on PubMed
Search for more literature for 1.13.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
A112D the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
A112G the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
A112I the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum
A112S the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
salicylate
-
 Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 2,5-Dihydroxybenzoate at pH 8.0 and 25°C Corynebacterium glutamicum
0.99
-
 5-Fluorosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
2.7
-
 5-Aminosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,5-dihydroxybenzoate + O2 Corynebacterium glutamicum the enzyme is highly specific for 2,5-dihydroxybenzoate maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2 Corynebacterium glutamicum ATCC 13032 the enzyme is highly specific for 2,5-dihydroxybenzoate maleylpyruvate
-
 ?

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NLB9
-
-
Corynebacterium glutamicum ATCC 13032 Q8NLB9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dihydroxybenzoate + O2 the enzyme is highly specific for 2,5-dihydroxybenzoate Corynebacterium glutamicum maleylpyruvate
-
 ?
2,5-dihydroxybenzoate + O2 the enzyme is highly specific for 2,5-dihydroxybenzoate Corynebacterium glutamicum ATCC 13032 maleylpyruvate
-
 ?
5-aminosalicylate + O2
-
 Corynebacterium glutamicum ?
-
 ?
5-aminosalicylate + O2
-
 Corynebacterium glutamicum ATCC 13032 ?
-
 ?
5-fluorosalicylate + O2
-
 Corynebacterium glutamicum ?
-
 ?
5-fluorosalicylate + O2
-
 Corynebacterium glutamicum ATCC 13032 ?
-
 ?
additional information no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate Corynebacterium glutamicum ?
-
 ?
additional information no activity with salicylate, 3-methylsalicylate, 4-methylsalicylate, 5-methylsalicylate, 3-bromosalicylate, 4-bromosalicylate, 5-iodosalicylate, and 1-hydroxy-2-naphthoate Corynebacterium glutamicum ATCC 13032 ?
-
 ?

Synonyms

Synonyms Comment Organism
GDO
-
 Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
 5-Fluorosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
1.1
-
 5-Aminosalicylate at pH 8.0 and 25°C Corynebacterium glutamicum
2310
-
 2,5-Dihydroxybenzoate at pH 8.0 and 25°C Corynebacterium glutamicum