Cloned (Comment) | Organism |
---|---|
gene narI, DNA and amino acid sequence determination and analysis of the gentisate catabolic gene cluster. Among the 13 complete open reading frames deduced from this fragment, three narIKL encode the enzymes involved in the reactions of gentisate catabolism, of these, NarI is gentisate 1,2-dioxygenase which converts gentisate to maleylpyruvate. Genes narI, narK, and narL genes are transcribed as a single operon only in the presence of naphthalene. Sequence comparisons, overview. Expression in Escherichia coli strain Rosetta (DE3) pLysS | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | Rhodococcus sp. | - |
maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | Rhodococcus sp. NCIMB 12038 | - |
maleylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | E7CYQ0 | gene narI | - |
Rhodococcus sp. NCIMB 12038 | E7CYQ0 | gene narI | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain NCIMB 12038 utilizes naphthalene as a sole source of carbon and energy | Rhodococcus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
145 | - |
recombinant enzyme, pH 7.4, 30°C | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Rhodococcus sp. | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Rhodococcus sp. NCIMB 12038 | maleylpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDO | - |
Rhodococcus sp. |
NarI | - |
Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rhodococcus sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | narI is involved in salicylate, i.e. 3-hydroxybenzoate, and gentisate catabolism, organization of the 3HBA-gentisate catabolic cluster, overview | Rhodococcus sp. |
additional information | strain NCIMB 12038 utilizes naphthalene as a sole source of carbon and energy, and degrades naphthalene via salicylate and gentisate. The strain can also use salicylate, i.e. 3-hydroxybenzoate, as sole source of energy and carbon | Rhodococcus sp. |