Cloned (Comment) | Organism |
---|---|
gene bphC, expressionin Escherichia coli strain BL21 (DE3) | Dyella ginsengisoli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | usage of the enzyme for construction of a catechol biosensor, method development involving PlackettBurman design and response surface methodology, overview. Maximal specific activity of BphC_LA-4 is about 0.58 U/mg with catechol as substrate. the BphC_LA-4 enzyme electrode prepared by SiO2 solgel shows good response to all these three catecholic compounds | Dyella ginsengisoli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dyella ginsengisoli | - |
gene bphC | - |
Dyella ginsengisoli LA-4 | - |
gene bphC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Dyella ginsengisoli | ? | - |
? | |
3-methylcatechol + O2 | - |
Dyella ginsengisoli LA-4 | ? | - |
? | |
4-methylcatechol + O2 | - |
Dyella ginsengisoli | ? | - |
? | |
4-methylcatechol + O2 | - |
Dyella ginsengisoli LA-4 | ? | - |
? | |
catechol + O2 | - |
Dyella ginsengisoli | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Dyella ginsengisoli LA-4 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
Dyella ginsengisoli |
BphC | - |
Dyella ginsengisoli |
BphC_LA-4 | - |
Dyella ginsengisoli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Dyella ginsengisoli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Dyella ginsengisoli |
General Information | Comment | Organism |
---|---|---|
additional information | analysis of the interaction between BphC_LA-4 and catecholic substrates by homology modeling and molecular docking, model of the BphC_LA-4-catechol complex, overview. BphC_LA-4 presents lower binding affinity towards 4-methylcatechol in comparison with 3-methylcatechol and catechol. A BphC_LA-4 enzyme electrode prepared by SiO2 sol-gel shows good response to all these three catecholic compounds | Dyella ginsengisoli |