Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Ralstonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | - |
- |
- |
Ralstonia sp. SBUG 290 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
shows 82% inactivation after 15 h at room temperature in Tris buffer (50 mM, pH 7.5) | Ralstonia sp. |
Purification (Comment) | Organism |
---|---|
Mono Q HR column chromatography and HiLoad 16/60 Superdex 75 gel filtration | Ralstonia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no substrate specificity for 3,4-dihydroxybiphenyl, 2,3-dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol | Ralstonia sp. |
0.17 | - |
using 4-methylcatechol as substrate | Ralstonia sp. |
0.33 | - |
using 1,2-dihydroxynaphthalene as substrate | Ralstonia sp. |
0.33 | - |
using catechol as substrate | Ralstonia sp. |
0.52 | - |
cell extract | Ralstonia sp. |
1.88 | - |
using 1,2-dihydroxydibenzofuran as substrate | Ralstonia sp. |
2.19 | - |
using 3-isopropylcatechol as substrate | Ralstonia sp. |
3.75 | - |
using 3-methylcatechol as substrate | Ralstonia sp. |
4.79 | - |
using 2,3-dihydroxybiphenyl as substrate | Ralstonia sp. |
6.88 | - |
after 13.2fold purification | Ralstonia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-dihydroxydibenzofuran + O2 | - |
Ralstonia sp. | ? | - |
? | |
1,2-dihydroxynaphthalene + O2 | - |
Ralstonia sp. | ? | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Ralstonia sp. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Ralstonia sp. SBUG 290 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
3-isopropylcatechol + O2 | - |
Ralstonia sp. | 2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ? | - |
? | |
3-methylcatechol + O2 | - |
Ralstonia sp. | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
3-methylcatechol + O2 | - |
Ralstonia sp. SBUG 290 | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Ralstonia sp. | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
4-methylcatechol + O2 | - |
Ralstonia sp. SBUG 290 | 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
Ralstonia sp. | ? | - |
? | |
catechol + O2 | - |
Ralstonia sp. SBUG 290 | ? | - |
? | |
additional information | no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol | Ralstonia sp. | ? | - |
? | |
additional information | no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol | Ralstonia sp. SBUG 290 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxybiphenyl-1,2-dioxygenase | - |
Ralstonia sp. |
2,3-diOH-biphenyl 1,2-dioxygenase | - |
Ralstonia sp. |
BphC | - |
Ralstonia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | for meta-cleavage of 1,2-dihydroxydibenzofuran | Ralstonia sp. |
30 | - |
for meta-cleavage of 2,3-dihydroxybiphenyl | Ralstonia sp. |