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Literature summary for 1.13.11.39 extracted from

  • Wesche, J.; Hammer, E.; Becher, D.; Burchhardt, G.; Schauer, F.
    The bphC gene-encoded 2,3-dihydroxybiphenyl-1,2-dioxygenase is involved in complete degradation of dibenzofuran by the biphenyl-degrading bacterium Ralstonia sp. SBUG 290 (2005), J. Appl. Microbiol., 98, 635-645.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Ralstonia sp.
-
-
-
Ralstonia sp. SBUG 290
-
-
-

Oxidation Stability

Oxidation Stability Organism
shows 82% inactivation after 15 h at room temperature in Tris buffer (50 mM, pH 7.5) Ralstonia sp.

Purification (Commentary)

Purification (Comment) Organism
Mono Q HR column chromatography and HiLoad 16/60 Superdex 75 gel filtration Ralstonia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
no substrate specificity for 3,4-dihydroxybiphenyl, 2,3-dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol Ralstonia sp.
0.17
-
using 4-methylcatechol as substrate Ralstonia sp.
0.33
-
using 1,2-dihydroxynaphthalene as substrate Ralstonia sp.
0.33
-
using catechol as substrate Ralstonia sp.
0.52
-
cell extract Ralstonia sp.
1.88
-
using 1,2-dihydroxydibenzofuran as substrate Ralstonia sp.
2.19
-
using 3-isopropylcatechol as substrate Ralstonia sp.
3.75
-
using 3-methylcatechol as substrate Ralstonia sp.
4.79
-
using 2,3-dihydroxybiphenyl as substrate Ralstonia sp.
6.88
-
after 13.2fold purification Ralstonia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dihydroxydibenzofuran + O2
-
Ralstonia sp. ?
-
?
1,2-dihydroxynaphthalene + O2
-
Ralstonia sp. ?
-
?
2,3-dihydroxybiphenyl + O2
-
Ralstonia sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
2,3-dihydroxybiphenyl + O2
-
Ralstonia sp. SBUG 290 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
3-isopropylcatechol + O2
-
Ralstonia sp. 2-hydroxy-7-methyl-6-oxo-octa-2,4-dienoate + ?
-
?
3-methylcatechol + O2
-
Ralstonia sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
3-methylcatechol + O2
-
Ralstonia sp. SBUG 290 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
4-methylcatechol + O2
-
Ralstonia sp. 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
?
4-methylcatechol + O2
-
Ralstonia sp. SBUG 290 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
?
catechol + O2
-
Ralstonia sp. ?
-
?
catechol + O2
-
Ralstonia sp. SBUG 290 ?
-
?
additional information no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol Ralstonia sp. ?
-
?
additional information no substrate specificity for 3,4-dihydroxybiphenyl, 2,3 dihydroxydibenzofuran, 3-chlorocatechol, 4-chlorocatechol, and pyrogallol Ralstonia sp. SBUG 290 ?
-
?

Synonyms

Synonyms Comment Organism
2,3-dihydroxybiphenyl-1,2-dioxygenase
-
Ralstonia sp.
2,3-diOH-biphenyl 1,2-dioxygenase
-
Ralstonia sp.
BphC
-
Ralstonia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 25 for meta-cleavage of 1,2-dihydroxydibenzofuran Ralstonia sp.
30
-
for meta-cleavage of 2,3-dihydroxybiphenyl Ralstonia sp.