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Literature summary for 1.13.11.39 extracted from

  • Hatta, T.; Mukerjee-Dhar, G.; Damborsky, J.; Kiyohara, H.; Kimbara, K.
    Characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a polychlorinated biphenyl- and naphthalene-degrading Bacillus sp. JF8 (2003), J. Biol. Chem., 278, 21483-21492.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000095
-
2,3-Dihydroxybiphenyl 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
0.001
-
3-methylcatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
0.025
-
4-Chlorocatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
0.1
-
catechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
0.11
-
4-methylcatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Manganese enzyme contains 4.0-4.8 manganese atoms per enzyme molecule, depending on the batch Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
4 * 35000, SDS-PAGE Bacillus sp. (in: Bacteria)
125000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q8GR45 JF8
-
Bacillus sp. (in: Bacteria) JF8 Q8GR45 JF8
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl, Phenyl-Sepharose, Mono Q Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.47
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybiphenyl + O2
-
Bacillus sp. (in: Bacteria) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
2,3-dihydroxybiphenyl + O2
-
Bacillus sp. (in: Bacteria) JF8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
3-methylcatechol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
3-methylcatechol + O2
-
Bacillus sp. (in: Bacteria) JF8 ?
-
?
4-chlorocatechol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
4-chlorocatechol + O2
-
Bacillus sp. (in: Bacteria) JF8 ?
-
?
4-methylcatechol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
4-methylcatechol + O2
-
Bacillus sp. (in: Bacteria) JF8 ?
-
?
catechol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
catechol + O2
-
Bacillus sp. (in: Bacteria) JF8 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 35000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
BphC_JF8
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
no loss of activity after 1 h Bacillus sp. (in: Bacteria)
70
-
25% loss of activity after 1 h Bacillus sp. (in: Bacteria)
80
-
approx. 60% loss of activity after 30 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Bacillus sp. (in: Bacteria)