Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000095 | - |
2,3-Dihydroxybiphenyl | 60°C, pH 7.5 | Bacillus sp. (in: Bacteria) | |
0.001 | - |
3-methylcatechol | 60°C, pH 7.5 | Bacillus sp. (in: Bacteria) | |
0.025 | - |
4-Chlorocatechol | 60°C, pH 7.5 | Bacillus sp. (in: Bacteria) | |
0.1 | - |
catechol | 60°C, pH 7.5 | Bacillus sp. (in: Bacteria) | |
0.11 | - |
4-methylcatechol | 60°C, pH 7.5 | Bacillus sp. (in: Bacteria) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | enzyme contains 4.0-4.8 manganese atoms per enzyme molecule, depending on the batch | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
125000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q8GR45 | JF8 | - |
Bacillus sp. (in: Bacteria) JF8 | Q8GR45 | JF8 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl, Phenyl-Sepharose, Mono Q | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.47 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybiphenyl + O2 | - |
Bacillus sp. (in: Bacteria) | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
3-methylcatechol + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
3-methylcatechol + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | ? | - |
? | |
4-chlorocatechol + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
4-chlorocatechol + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | ? | - |
? | |
4-methylcatechol + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
4-methylcatechol + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | ? | - |
? | |
catechol + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
catechol + O2 | - |
Bacillus sp. (in: Bacteria) JF8 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 35000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
BphC_JF8 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no loss of activity after 1 h | Bacillus sp. (in: Bacteria) |
70 | - |
25% loss of activity after 1 h | Bacillus sp. (in: Bacteria) |
80 | - |
approx. 60% loss of activity after 30 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Bacillus sp. (in: Bacteria) |