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Literature summary for 1.13.11.39 extracted from

  • Vaillancourt, F.H.; Han, S.; Fortin, P.D.; Bolin, J.T.; Eltis, L.D.
    Molecular basis for the stabilization and inhibition of 2,3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol (1998), J. Biol. Chem., 273, 34887-34895.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Burkholderia cepacia
strain KKS102 Pseudomonas sp.

General Stability

General Stability Organism
tert-butanol, glycerol, isopropylalcohol and ethanol stabilizes the enzyme Burkholderia cepacia

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dihydroxybiphenyl reversible substrate inhibition Burkholderia cepacia
2,3-Dihydroxybiphenyl
-
Pseudomonas sp.
3-chlorocatechol
-
Burkholderia cepacia
3-ethylcatechol reversible substrate inhibition Burkholderia cepacia
acetone competitive inhibition Burkholderia cepacia
ethanol
-
Burkholderia cepacia
glycerol
-
Burkholderia cepacia
isopropylalcohol
-
Burkholderia cepacia
tert-butanol
-
Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
2,3-Dihydroxybiphenyl
-
Burkholderia cepacia
1.28
-
O2
-
Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl-2,3-diol + O2 Pseudomonas sp.
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 Burkholderia cepacia
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2 Burkholderia cepacia LB400
-
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia LB400
-
-
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
431
-
-
Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-ethylcatechol + O2
-
Burkholderia cepacia 2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate
-
?
3-ethylcatechol + O2
-
Burkholderia cepacia LB400 2-hydroxy-7-methyl-6-oxoocta-2,4-dienoate
-
?
3-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
3-methylcatechol + O2
-
Burkholderia cepacia 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
3-methylcatechol + O2
-
Burkholderia cepacia LB400 2-hydroxy-6-oxohepta-2,4-dienoate
-
?
biphenyl-2,3-diol + O2
-
Pseudomonas sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2
-
Burkholderia cepacia 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
biphenyl-2,3-diol + O2
-
Burkholderia cepacia LB400 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
?
catechol + O2
-
Pseudomonas sp. ?
-
?
catechol + O2
-
Burkholderia cepacia ?
-
?
catechol + O2
-
Burkholderia cepacia LB400 ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.22
-
3-chlorocatechol
-
Burkholderia cepacia
3
-
2,3-Dihydroxybiphenyl reversible substrate inhibition Burkholderia cepacia
5.2
-
tert-butanol catechol as substrate Burkholderia cepacia
7.3
-
tert-butanol 2,3-dihydroxybiphenyl as substrate Burkholderia cepacia
9.9
-
tert-butanol 3-methylcatechol as substrate Burkholderia cepacia
10.5
-
isopropylalcohol
-
Burkholderia cepacia
11
-
3-ethylcatechol
-
Burkholderia cepacia
13
-
acetone
-
Burkholderia cepacia
35
-
ethanol
-
Burkholderia cepacia