Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pimelobacter simplex |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor difffusion from a solution containing 2 M ammonium sulfate, 4% polyethylene glycol 400, 100 mM Hepes, pH 7.5, drops consist of 0.004 ml of 20 mg/ml protein solution and 0.006 ml reservoir solution equilibrated against 0.05 ml of reservoir solution, crystals diffract to 1.75 A | Pimelobacter simplex |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
catechol | - |
Pimelobacter simplex |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
Benzene-1,2,4-triol | - |
Pimelobacter simplex |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | required for activity | Pimelobacter simplex |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene-1,2,4-triol + O2 | Pimelobacter simplex | - |
3-hydroxy-cis,cis-muconate | - |
? | |
benzene-1,2,4-triol + O2 | Pimelobacter simplex 3E | - |
3-hydroxy-cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pimelobacter simplex | Q5PXQ6 | 3E | - |
Pimelobacter simplex 3E | Q5PXQ6 | 3E | - |
Purification (Comment) | Organism |
---|---|
recombinant 1,2-HQD | Pimelobacter simplex |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene-1,2,4-triol + O2 | - |
Pimelobacter simplex | 3-hydroxy-cis,cis-muconate | - |
? | |
benzene-1,2,4-triol + O2 | - |
Pimelobacter simplex 3E | 3-hydroxy-cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,2-HQD | - |
Pimelobacter simplex |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
Benzene-1,2,4-triol | - |
Pimelobacter simplex |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
catechol | approximate value | Pimelobacter simplex |