Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Fe(NH4)2(SO4)2 | 0.00001-0.000001 mM | Azotobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Tiron | - |
Azotobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 0.18 mol per mol enzyme | Azotobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34250 | - |
2 * 34250, SDS-PAGE | Azotobacter sp. |
58000 | - |
gel filtration | Azotobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzene-1,2,4-triol + O2 | Azotobacter sp. | - |
3-hydroxy-cis-cis-muconate | - |
? | |
benzene-1,2,4-triol + O2 | Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 | - |
3-hydroxy-cis-cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter sp. | - |
grown with 2,4,6-trichlorophenol | - |
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 | - |
grown with 2,4,6-trichlorophenol | - |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.06 | - |
reactivated with 0.01 mM Fe2+ | Azotobacter sp. |
Storage Stability | Organism |
---|---|
-80°C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day | Azotobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-chlorohydroxyquinol + O2 | 33.5% relative activity of that measured with hydroxyquinol | Azotobacter sp. | 2-chloromaleylacetate | - |
? | |
6-chlorohydroxyquinol + O2 | 33.5% relative activity of that measured with hydroxyquinol | Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 | 2-chloromaleylacetate | - |
? | |
benzene-1,2,4-triol + O2 | - |
Azotobacter sp. | maleylacetate | maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate | ? | |
benzene-1,2,4-triol + O2 | - |
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 | maleylacetate | maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate | ? | |
benzene-1,2,4-triol + O2 | - |
Azotobacter sp. | 3-hydroxy-cis-cis-muconate | - |
? | |
benzene-1,2,4-triol + O2 | - |
Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 | 3-hydroxy-cis-cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34250, SDS-PAGE | Azotobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
in succinate buffer | Azotobacter sp. |