BRENDA - Enzyme Database show
show all sequences of 1.13.11.36

Catechol 2,3-dioxygenase from pyrazon-degrading bacteria

Muller, R.; Haug, S.; Eberspcher, J.; Lingens, F.; Hoppe-Seyler's Z. Physiol. Chem. 358, 797-805 (1977)

Data extracted from this reference:

General Stability
General Stability
Organism
high osmotic sensitivity
Phenylobacterium immobile
Inhibitors
Inhibitors
Commentary
Organism
Structure
High ionic strength
-
Phenylobacterium immobile
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
3-Methylpyrocatechol
-
Phenylobacterium immobile
0.16
-
4-Methylpyrocatechol
-
Phenylobacterium immobile
0.2
-
5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone
-
Phenylobacterium immobile
0.66
-
pyrocatechol
-
Phenylobacterium immobile
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Fe2+
Phenylobacterium immobile
Fe2+
required for activity
Phenylobacterium immobile
Zn2+
can partially replace Fe2+
Phenylobacterium immobile
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
6 * 34000, SDS-PAGE
Phenylobacterium immobile
200000
-
analytical ultracentrifugation
Phenylobacterium immobile
220000
-
gel filtration
Phenylobacterium immobile
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
Phenylobacterium immobile
enzyme of pyrazone metabolism
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phenylobacterium immobile
-
DSM 1986
-
Purification (Commentary)
Commentary
Organism
-
Phenylobacterium immobile
Storage Stability
Storage Stability
Organism
-20C, stable for months
Phenylobacterium immobile
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-methylpyrocatechol + O2
-
439432
Phenylobacterium immobile
2-hydroxy-3-methylmuconic acid semialdehyde
-
-
-
?
4-methylpyrocatechol + O2
-
439432
Phenylobacterium immobile
2-hydroxy-3-methylmuconic acid semialdehyde
-
-
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
-
439432
Phenylobacterium immobile
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
enzyme of pyrazone metabolism
439432
Phenylobacterium immobile
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
-
?
catechol + O2
-
439432
Phenylobacterium immobile
2-hydroxymuconic acid semialdehyde
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 34000, SDS-PAGE
Phenylobacterium immobile
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Phenylobacterium immobile
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Phenylobacterium immobile
General Stability (protein specific)
General Stability
Organism
high osmotic sensitivity
Phenylobacterium immobile
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
High ionic strength
-
Phenylobacterium immobile
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
3-Methylpyrocatechol
-
Phenylobacterium immobile
0.16
-
4-Methylpyrocatechol
-
Phenylobacterium immobile
0.2
-
5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone
-
Phenylobacterium immobile
0.66
-
pyrocatechol
-
Phenylobacterium immobile
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
can partially replace Fe2+
Phenylobacterium immobile
Fe2+
required for activity
Phenylobacterium immobile
Zn2+
can partially replace Fe2+
Phenylobacterium immobile
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
6 * 34000, SDS-PAGE
Phenylobacterium immobile
200000
-
analytical ultracentrifugation
Phenylobacterium immobile
220000
-
gel filtration
Phenylobacterium immobile
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
Phenylobacterium immobile
enzyme of pyrazone metabolism
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Phenylobacterium immobile
Storage Stability (protein specific)
Storage Stability
Organism
-20C, stable for months
Phenylobacterium immobile
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-methylpyrocatechol + O2
-
439432
Phenylobacterium immobile
2-hydroxy-3-methylmuconic acid semialdehyde
-
-
-
?
4-methylpyrocatechol + O2
-
439432
Phenylobacterium immobile
2-hydroxy-3-methylmuconic acid semialdehyde
-
-
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
-
439432
Phenylobacterium immobile
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
-
?
5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
enzyme of pyrazone metabolism
439432
Phenylobacterium immobile
5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
-
-
?
catechol + O2
-
439432
Phenylobacterium immobile
2-hydroxymuconic acid semialdehyde
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 34000, SDS-PAGE
Phenylobacterium immobile
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Phenylobacterium immobile
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Phenylobacterium immobile
Other publictions for EC 1.13.11.36
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439434
Schmitt
-
One-step purification of chlor ...
Phenylobacterium immobile
J. Immunol. Methods
68
263-267
1984
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439435
Schmitt
Chloridazon-catechol dioxygena ...
Phenylobacterium immobile, Phenylobacterium sp.
Hoppe-Seyler's Z. Physiol. Chem.
365
143-150
1984
-
-
-
-
-
-
-
-
-
2
-
2
-
6
-
-
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439433
Muller
A novel non-heme iron-containi ...
Phenylobacterium immobile
Eur. J. Biochem.
125
579-584
1982
1
-
-
1
-
-
-
-
-
1
-
1
-
3
1
-
-
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
439432
Muller
Catechol 2,3-dioxygenase from ...
Phenylobacterium immobile
Hoppe-Seyler's Z. Physiol. Chem.
358
797-805
1977
-
-
-
-
-
1
1
4
-
3
3
1
-
1
-
-
1
-
-
-
-
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
4
-
3
3
1
-
-
-
1
-
-
-
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-