Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
additional information | the enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene ALOX5, located on chromosome 10q11.21, it spans a region of 71.9 kbp and consists of 13 introns named A-M and 14 exons, semiquantitative PCR enzyme expression analysis. Recombinant expression of enzyme mutant 5-LODELTA4 in HEK-293T cells from expression plasmid, recombinant expression of soluble 5-LODELTA4 mutant and of wild-type enzyme 5-LO in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | identification of a protein isoform of human 5-LO that lacks exon 4, termed 5-LODELTA4, identified in cells of lymphoid origin, namely the Burkitt lymphoma cell lines Raji and BL41 as well as primary B and T cells, semiquantitative PCR enzyme expression analysis. Deletion of exon 4 does not shift the reading frame and therefore the mRNA is not subjected to non-mediated mRNA decay. Enzyme mutant 5-LODELTA4 isoform is a stable protein in eukaryotic cells. The isoform itself lacks canonical enzymatic activity as it misses the non-heme iron but it still retains ATP-binding affinity. Whilst the catalytic domain of wild-type 5-LO is destabilized by calcium, addition of calcium has no influence on the catalytic domain of 5-LODELTA4. The enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | whilst the catalytic domain of wild-type 5-LO is destabilized by calcium, addition of calcium has no influence on the catalytic domain of 5-LODELTA4 | Homo sapiens | |
Fe2+ | a non-heme iron that is required for catalytic activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | Homo sapiens | - |
(5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09917 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble 5-LODELTA4 mutant and wild-type enzyme 5-LO from Escherichia coli strain BL21(DE3) by ATP affinity chromatography and anion exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte | - |
Homo sapiens | - |
BL-41 cell | - |
Homo sapiens | - |
Burkitt lymphoma cell | - |
Homo sapiens | - |
dendritic cell | - |
Homo sapiens | - |
leukocyte | - |
Homo sapiens | - |
macrophage | - |
Homo sapiens | - |
mast cell | - |
Homo sapiens | - |
monocyte | - |
Homo sapiens | - |
additional information | 5-LO is mainly expressed in polymorphonuclear leukocytes, monocytes/macrophages, dendritic cells, mast cells and B cells | Homo sapiens | - |
RAJI cell | - |
Homo sapiens | - |
T-lymphocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Homo sapiens | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77900, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
5-lipoxygenase | - |
Homo sapiens |
5-LO | - |
Homo sapiens |
ALOX5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | a protein isoform of human 5-LO that lacks exon 4, termed 5-LODELTA4, lacks canonical enzymatic activity as it misses the non-heme iron but it still retains ATP-binding affinity. The enzyme mutant 5-LODELTA4 stimulates wild-type 5-LO activity and product formation at low protein concentrations | Homo sapiens |
physiological function | leukotrienes are inflammatory mediators that play a pivotal role in many diseases like asthma bronchiale, atherosclerosis and in various types of cancer. The key enzyme for generation of leukotrienes is the 5-lipoxygenase, 5-LO, that catalyzes the initial steps in the conversion of arachidonate to the instable epoxide leukotriene A4 (LTA4) via the intermediate 5(S)-hydroperoxy-6,8,11,14-(E,Z,Z,Z)-eicosatetraenoic acid (5-HpETE). LTA4 can then be metabolized by the LTA4 hydrolase to the potent chemoattractant and leukocyte activator LTB4 or conjugated with glutathione to the cysteinyl leukotriene LTC4 by LTC4 synthase | Homo sapiens |