Any feedback?
Literature summary for 1.13.11.34 extracted from
- Molecular cloning and functional characterization of arachidonate 5-lipoxygenase (Alox5), and its expression in response to the ratio of linolenic acid to linoleic acid in diets of large yellow croaker (Larmichthys crocea) (2016), Comp. Biochem. Physiol. B, 201, 21-28 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Alox5, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative realtime PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Larimichthys crocea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | Larimichthys crocea | epoxidation | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? |  |
| arachidonate + O2 | Larimichthys crocea | hydroperoxidation | (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Larimichthys crocea | A0A075C1I3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Larimichthys crocea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Larimichthys crocea | - |
| heart | - |
Larimichthys crocea | - |
| intestine | - |
Larimichthys crocea | - |
| kidney | high expression level. In the kidney, the expression levels of Alox5 of fish fed diets with low dietary linolenic acid/linoleic acid (0.03-0.06) are significantly higher than those of fish fed diets with high dietary linolenic acid/linoleic acid (0.45-1.51) | Larimichthys crocea | - |
| liver | in the liver, the Alox5 mRNA expression levels significantly increase to the maximum when the dietary linolenic acid/linoleic acid increases from 0.03 to 0.06, and then significantly decreases with dietary linolenic acid/linoleic acid increased to 1.51 | Larimichthys crocea | - |
| muscle | low expression level | Larimichthys crocea | - |
| spleen | - |
Larimichthys crocea | - |
| stomach | - |
Larimichthys crocea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | epoxidation | Larimichthys crocea | (5S,6S,7E,9E,11Z,14Z)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | - |
? | |
| arachidonate + O2 | hydroperoxidation | Larimichthys crocea | (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 78060, sequence calculation | Larimichthys crocea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALOX5 | - |
Larimichthys crocea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Larimichthys crocea | sequence calculation | - |
5.83 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Larimichthys crocea | in the liver, the Alox5 mRNA expression levels significantly increase to the maximum when the dietary linolenic acid/linoleic acid increases from 0.03 to 0.06, and then significantly decreases with dietary linolenic acid/linoleic acid increased to 1.51. In the kidney, the expression levels of Alox5 of fish fed diets with low dietary linolenic acid/linoleic acid (0.03-0.06) are significantly higher than those of fish fed diets with high dietary linolenic acid/linoleic acid (0.45-1.51) | up |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
