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Literature summary for 1.13.11.34 extracted from

  • Walther, M.; Hofheinz, K.; Vogel, R.; Roffeis, J.; Kuehn, H.
    The N-terminal beta-barrel domain of mammalian lipoxygenases including mouse 5-lipoxygenase is not essential for catalytic activity and membrane binding but exhibits regulatory functions (2011), Arch. Biochem. Biophys., 516, 1-9.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidyl choline addition increases catalytic activity of wild-type nzyme by 50% Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Mus musculus

Protein Variants

Protein Variants Comment Organism
DELTA1-114 D114 truncation mutant shows 6.8% residual activity compared to wild-type, Km (arachidonate) increased compared to wild-type. Vmax: 9.3% compared to wild-type (100%). Addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties Mus musculus
DELTA1-125 I125 truncation mutant shows 1.8% residual activity compared to wild-type, addition of phosphatidyl choline or Ca2+ increases catalytic activity only by 11% compared to 50% in wild-type. Truncated mutant exhibits impaired membrane binding properties Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
arachidonate wild-type, pH and temperature not specified in the publication Mus musculus
0.06
-
arachidonate mutant DELTA1-114, pH and temperature not specified in the publication Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of Ca2+ increases catalytic activity of wild-type nzyme by 50% Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
Mus musculus (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
5-LOX
-
Mus musculus