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Literature summary for 1.13.11.34 extracted from

  • Radmark, O.; Samuelsson, B.
    5-Lipoxygenase: mechanisms of regulation (2009), J. Lipid Res., 50 Suppl, S40-S45.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
1,25(OH)2D3 gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells Homo sapiens
CLP can upregulate and modulate the 5-lipoxygenase pathway in vitro Homo sapiens
additional information in the mouse 5-lipoxygenase gene there are no GC-boxes in tandem, the core promoter contains only one Sp1/3 binding site Mus musculus
transforming growth factor beta gives a strong (100fold) upregulation of 5-lipoxygenase protein in differentiating Mono Mac 6 cells Homo sapiens

Application

Application Comment Organism
drug development gender difference in biosynthesis of leukotrienes (inflammatory mediators) may lead to new possibilities regarding development and use of 5-lipoxygenase inhibitors Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ABT-761
-
Homo sapiens
cholesterol addition to a membrane preperation in vitro reduces 5-lipoxygenase activity by half. Cholesterol sulfate can inhibit 5-lipoxygenase in intact cells Homo sapiens
mithramycin reduces endogenous 5-lipoxygenase in Mono Mac 6 cell, which blocks GC-boxes in the proximal part of the 5-lipoxygenase gene promoter Homo sapiens
Zileutin
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol resides in the cytosol of resting neutrophils from females Homo sapiens 5829
-
membrane translocates from the cytosol to the membrane upon stimulation in female neutrophils Homo sapiens 16020
-
nucleus resides in a nuclear soluble compartment associated with chromatin Rattus norvegicus 5634
-
nucleus resides in a nuclear soluble compartment associated with chromatin. In male neutrophils, a substantial part of 5-lipoxygenase is located at the perinuclear region already in resting cells, only marginally redistributes upon stimulation Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Homo sapiens
Fe2+
-
Homo sapiens
Mg2+ activates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
B-lymphocyte
-
Homo sapiens
-
basophilic leukemia cell
-
Rattus norvegicus
-
dendritic cell
-
Homo sapiens
-
eosinophil
-
Homo sapiens
-
foam cell atherosclerotic tissue Homo sapiens
-
HL-60 cell the 5-lipoxygenase core promoter is unmethylated, expression of 5-lipoxygenase protein upon differentiation Homo sapiens
-
leukocyte
-
Homo sapiens
-
macrophage
-
Homo sapiens
-
mast cell
-
Homo sapiens
-
Mono-Mac-6 cell
-
Homo sapiens
-
monocyte
-
Homo sapiens
-
neutrophil
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + O2
-
Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
5-lipoxygenase
-
Mus musculus
5-lipoxygenase
-
Homo sapiens
5-lipoxygenase
-
Rattus norvegicus
5-LO
-
Mus musculus
5-LO
-
Homo sapiens
5-LO
-
Rattus norvegicus

Expression

Organism Comment Expression
Homo sapiens DNA methylation is responsible for suppression of 5-lipoxygenase expression in most cell types down
Homo sapiens aberrant methylation may lead to upregulated 5-lipoxygenase expression in tumor cells. The 5-lipoxygenase core promoter is completely methylated in cell lines U-937 and HL-60TB, which do not express 5-lipoxygenase. Treatment with the demethylating agent 5-aza-2'deoxycytidine restores 5-lipoxygenase expression up

General Information

General Information Comment Organism
physiological function catalyzes two steps in biosynthesis of leukotrienes, a group of lipid mediators of inflammation derived from arachidonic acid, which are used as antagonists in treatment of asthma. A potential role also in atherosclerosis Homo sapiens