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Literature summary for 1.13.11.34 extracted from

  • Pufahl, R.A.; Kasten, T.P.; Hills, R.; Gierse, J.K.; Reitz, B.A.; Weinberg, R.A.; Masferrer, J.L.
    Development of a fluorescence-based enzyme assay of human 5-lipoxygenase (2007), Anal. Biochem., 364, 204-212.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
from adipose tissue, expression of the enzyme in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, subcloning and propagation of the virus in Escherichia coli strain DH10 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CJ-13610
-
Homo sapiens
glutathione inhibition is increased in presence of glutathione peroxidase Homo sapiens
nordihydroguaiaretic acid
-
Homo sapiens
SC-756
-
Homo sapiens
ZD-2138
-
Homo sapiens
zileuton
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate Homo sapiens
-
leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
arachidonic acid + O2 Homo sapiens first committed step in the synthesis of leukotrienes (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
Homo sapiens
-
brain
-
Homo sapiens
-
cerebellum
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
Homo sapiens leukotriene A4 + H2O
-
?
(6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
Homo sapiens leukotriene A4 + H2O is unstable and metabolized to leukotriene B4 ?
2',7'-dichlorodihydrofluorescein diacetate + O2 development of a fluorescence assay for 5-LOX, overview Homo sapiens ?
-
?
arachidonic acid + O2
-
Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
?
arachidonic acid + O2 first committed step in the synthesis of leukotrienes Homo sapiens (6E,8Z,11Z,14Z)-(5S)-5-hydroperoxyeicosa-6,8,11,14-tetraenoate
-
?

Synonyms

Synonyms Comment Organism
5-lipoxygenase
-
Homo sapiens
5-LOX
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
enzyme inhibition in presence of inhibitor and glutathione peroxidase, overview Homo sapiens additional information
0.00009
-
pH 7.5, 37°C, assay method via fluorescence measurement Homo sapiens nordihydroguaiaretic acid
0.00011 0.00012 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement Homo sapiens ZD-2138
0.00012 0.00092 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement Homo sapiens zileuton
0.00013
-
pH 7.5, 37°C, assay method via fluorescence measurement Homo sapiens SC-756
0.00115 0.00155 pH 7.5, 37°C, dependent on assay method via fluorescence or UV measurement, kinetics, overview Homo sapiens CJ-13610